A REGION IMMEDIATELY ADJACENT TO THE ORIGIN OF REPLICATION OF BOVINE PAPILLOMA-VIRUS TYPE-1 INTERACTS INVITRO WITH THE NUCLEAR MATRIX

被引：10

作者：

ADOM, JN ^{[1
]}

RICHARDFOY, H ^{[1
]}

机构：

[1] HOP KREMLIN BICETRE, UNITE RECH COMMUN HORMONALES,INSERM,U33, 78 RUE GEN LECLERC, F-94275 LE KREMLIN BICETRE, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 01期

关键词：

D O I：

10.1016/0006-291X(91)90949-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the interaction of the 69% transforming fragment of the Bovine Papilloma Virus type 1 (BPV1) with the nuclear matrix from 1361.5 cells (NIH-3T3 cells transformed by a BPV chimeric construct). in vitro studies performed with end-labelled DNA fragments and nuclear matrices prepared using a high-salt extraction procedure demonstrate the binding of a 672 bp fragment adjacent to the viral origin of replication and containing the plasmid maintenance sequence (PMS-1). This fragment can be cleaved into two pieces (393 and 279 bp), both interacting equally well with the nuclear matrix. This indicates that a least two regions of the 672 bp DNA fragment are involved in the interaction. © 1991.

引用

页码：479 / 485

页数：7

共 21 条

[1]

ADOM J, UNPUB

[2] CHROMOSOMAL ARS AND CEN ELEMENTS BIND SPECIFICALLY TO THE YEAST NUCLEAR SCAFFOLD [J].