INTRACELLULAR REDISTRIBUTION OF NEUROPEPTIDES AND SECRETORY PROTEINS DURING DIFFERENTIATION OF NEURONAL CELL-LINES

被引:59
作者
SUBURO, AM
WHEATLEY, SC
HORN, DA
GIBSON, SJ
JAHN, R
FISCHERCOLBRIE, R
WOOD, JN
LATCHMAN, DS
POLAK, JM
机构
[1] HAMMERSMITH HOSP, ROYAL POSTGRAD MED SCH, DEPT HISTOCHEM, DU CANE RD, LONDON W12 0NN, ENGLAND
[2] UNIV COLL & MIDDLESEX SCH MED, DEPT BIOCHEM, MED MOLEC BIOL, LONDON WC1 6DP, ENGLAND
[3] MAX PLANCK INST PSYCHIAT, DEPT NEUROCHEM, W-8033 MARTINSRIED, GERMANY
[4] UNIV INNSBRUCK, DEPT PHARMACOL, A-6020 INNSBRUCK, AUSTRIA
[5] SANDOZ INST MED RES, LONDON EC1E 6BN, ENGLAND
关键词
D O I
10.1016/0306-4522(92)90191-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have demonstrated that the mouse neuroblastoma N18Tg2 cell line and several clones of hybrid ND cells (ND7, ND9 and ND21), derived from the fusion of neonatal rat sensory neurons with that neuroblastoma, show immunostaining to protein gene product 9.5, neuropeptide Y, C-flanking peptide of neuropeptide Y, tyrosine hydroxylase and chromogranins. Synaptophysin could only be detected in ND cells. Immunoreactivities to substance P, calcitonin gene-related peptide, galanin and somatostatin could not be detected in any of these cell lines. After three days of incubation in a differentiation medium, cell processes of various lengths were observed both in neuroblastoma and ND cell cultures. In ND7 cells there was also a redistribution of neuropeptide Y and its C-flanking peptide to the tips of cell processes. The differentiation of cell processes was also accompanied by the appearance of immunostaining to rat chromogranins in their tips. In contrast, synaptophysin expression was found mainly in cell bodies. Neuropeptide Y, its C-flanking peptide and chromogranins have been associated with secretory granules, whereas synaptophysin is a marker for small synaptic-like vesicles. Therefore, our morphological findings further support and expand the view that these markers are primarily associated with different subcellular structures. Moreover, they indicate that the regulated secretory pathway associated with chromogranins is segregated into nerve processes at an early stage of differentiation, when the synaptophysin-associated pathway is not yet mature. ND7 cells thus provide a useful model system for studying changes in the distribution of neuropeptides, cytoskeletal elements and proteins associated with cell secretion during neuronal differentiation.
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页码:881 / 889
页数:9
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