DETECTION OF INTERSTITIAL INCREASE IN MACROPHAGES, CHARACTERISTIC OF ACUTE INTERSTITIAL REJECTION, IN ROUTINELY PROCESSED RENAL-ALLOGRAFT BIOPSIES USING THE MONOCLONAL-ANTIBODY KP1

被引：7

作者：

DOOPER, IMM

BOGMAN, MJJT

HOITSMA, AJ

MAASS, CN

ASSMANN, KJM

KOENE, RAP

机构：

[1] Department of Pathology, Division of Nephrology, University Hospital Nijmegen, Nijmegen, NL-6500 HB

[2] Department of Medicine, Division of Nephrology, University Hospital Nijmegen, Nijmegen, NL-6500 HB

来源：

TRANSPLANT INTERNATIONAL | 1992年 / 5卷 / 04期

关键词：

KIDNEY TRANSPLANTATION; BIOPSY; MONOCLONAL ANTIBODY; KIDNEY BIOPSY; KP1;

D O I：

10.1007/BF00336072

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Acute interstitial rejection (AIR) of renal allografts is accompanied by a characteristic peritubular increase in macrophages, which can be identified with the CD14 monoclonal antibody (mAb) WT14 in cryostat sections. Since frozen tissue is not always available, we tested whether this increase can also be demonstrated in Bouin-fixed, paraffin-embedded biopsies, using the CD68 anti-macrophage mAb KP1, which can also be applied to paraffin sections. Sections of 16 biopsies with AIR and 11 controls were stained with KP1. In 25 of the 27 biopsies, macrophages were strongly positive for KP1. Two AIR biopsies were completely negative, probably due to prolonged fixation. In the remaining 14 AIR biopsies, the number of KP1-positive cells was significantly higher than in the controls [1184 +/- 410 per mm2 (mean +/- SD) vs 112 +/- 126 per MM2]. We conclude that, especially in cases in which frozen tissue is not available, the demonstration of increased numbers of monocytes/macrophages with mAb KP1 can be a helpful adjunct in the histological diagnosis of AIR in routinely processed renal biopsies.

引用

页码：209 / 213

页数：5

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