ENHANCED RESULTS IN MOUSE AND HUMAN EMBRYO CULTURE USING A MODIFIED HUMAN TUBAL FLUID MEDIUM LACKING GLUCOSE AND PHOSPHATE

被引：121

作者：

QUINN, P ^{[1
]}

机构：

[1] SAN FERNANDO VALLEY FERTIL & REPROD CTR,TARZANA,CA 91356

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 02期

关键词：

GLUCOSE; EMBRYO DEVELOPMENT; IMPLANTATION RATE;

D O I：

10.1007/BF02211377

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Because various recent studies in both human and laboratory animals have indicated an influential role of glucose and phosphate in embryonic development, this study was initiated to assess the effect of culture medium lacking glucose and phosphate on human IVF. Results: Initial studies with mouse zygotes indicated a significant improvement in embryonic development in both a F1 hybrid and a random-bred ''two-cell block'' strain, using a modified HTF medium which contained EDTA and glutamine (Gln) and also with a similar medium which lacked glucose and phosphate. Subsequently, a prospective randomized trial of human IVF has begun in which infertile patients are sequentially assigned to have NF using one of three media. The three media are regular HTF, HTF containing EDTA and Gln (referred to as Basal HTF), and HTF with EDTA and Gln but devoid of glucose and phosphate ions (referred to as Basal XI HTF). Conclusions: Initial results indicate that Basal XI HTF medium, a modified form of HTF containing EDTA and glutamine but devoid of glucose and phosphate, greatly improves the in vitro development of mouse and human embryos over HTF and Basal HTF medium.

引用

页码：97 / 105

页数：9

共 33 条

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