HEAT-STABLE TOXIN FROM ESCHERICHIA-COLI ACTIVATES CHLORIDE CURRENT VIA CGMP-DEPENDENT PROTEIN-KINASE

被引：14

作者：

LIN, MQ

MACLEOD, K

GUGGINO, S

机构：

[1] JOHNS HOPKINS UNIV, DEPT MED, DIV GASTROENTEROL, BALTIMORE, MD 21205 USA

[2] JOHNS HOPKINS UNIV, DEPT NEUROSCI, BALTIMORE, MD USA

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 1995年 / 5卷 / 01期

关键词：

DIARRHEA; CHANNEL; T84; CELLS; KINASE; INTESTINE;

D O I：

10.1159/000154737

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Heat-stable toxin (STa) increases cyclic GMP (cGMP) in isolated intestinal cells and in T84 cells, a colonic secretory cell line. Whole-cell current recordings from patch clamp experiments show identical properties for currents activated by either STa or the cystic fibrosis transmembrane conductance regulator (CFTR) channel. STa-activated currents display a linear current-voltage relationship and a relative permeability sequence of Br > Cl > I. STa or 8-Br-cGMP-activated currents remain when 20 mu M Walsh inhibitor, a blocker of protein kinase A (PKA), is added in the pipette, suggesting that cGMP-dependent protein kinase (PKG) activates the currents. Intracellular addition of Rp-8-Br-cGMP, an agent that activates PKGII and inhibits PKGI and PKA, causes induction of a chloride conductance identical to that stimulated by STa. We conclude that STa activates CFTR by phosphorylation with cGMP-dependent protein kinase.

引用

收藏

页码：23 / 32

页数：10

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