EFFECT OF PHOSPHOLIPASE-C-GAMMA OVEREXPRESSION ON PDGF-INDUCED 2ND MESSENGERS AND MITOGENESIS

被引：170

作者：

MARGOLIS, B

ZILBERSTEIN, A

FRANKS, C

FELDER, S

KREMER, S

ULLRICH, A

RHEE, SG

SKORECKI, K

SCHLESSINGER, J

机构：

[1] UNIV TORONTO,MEMBRANE BIOL GRP,TORONTO M5S 1A1,ONTARIO,CANADA

[2] MAX PLANCK INST BIOCHEM,W-8033 MARTINSRIED,GERMANY

[3] NHLBI,BETHESDA,MD 20892

来源：

SCIENCE | 1990年 / 248卷 / 4955期

关键词：

D O I：

10.1126/science.2333512

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Platelet-derived growth factor (PDGF) stimulates phospholipase C (PLC) activity and the phosphorylation of the γ isozyme of PLC (PLC-γ) in vitro and in living cells. The role of PLC-γ in the phosphoinositide signaling pathway was addressed by examining the effect of overexpression of PLC-γ on cellular responses to PDGF. Overexpression of PLC-γ correlated with PDGF-induced tyrosine phosphorylation of PLC-γ and with PDGF-induced breakdown of phosphatidylinositol 4,5-bisphosphate (PIP 2). However, neither bradykinin- nor lysophosphatidic acid - induced phosphoinositide metabolism was enhanced in the transfected cells, suggesting that the G protein - coupled phosphoinositide responses to these ligands are mediated by other PLC isozymes. The enhanced PDGF-induced generation of inositol trisphosphate (IP3) did not enhance intracellular calcium signaling or influence PDGF-induced DNA synthesis. Thus, enzymes other than PLC-γ may limit PDGF-induced calcium signaling and DNA synthesis. Alternatively, PDGF-induced calcium signaling and DNA synthesis may use biochemical pathways other than phosphoinositide metabolism for signal transduction.

引用

页码：607 / 610

页数：4

共 29 条

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