COMPARISON OF A NEW OVOMUCOID AND A 2ND-GENERATION ALPHA-1-ACID GLYCOPROTEIN-BASED CHIRAL COLUMN FOR THE DIRECT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY RESOLUTION OF DRUG ENANTIOMERS

A new commercially-available glycoprotein chiral stationary phase (CSP) based on immobilized ovomucoid protein has been evaluated. This column has been quantitatively compared to the second-generation alpha-1-acid glycoprotein CSP for the direct resolution of enantiomers of sixteen commercially-available racemic drugs and eight proprietary drug candidates. The experimental protocol utilized simple aqueous-organic mobile phases with optimization schemes varying only in phosphate buffer concentration, pH and organic solvent modifier type and concentration. Cationic and anionic modifiers were not used to achieve separations due to concerns for consistent column performance. Column stability was monitored by comparing separation factors, resolution, and column efficiency following 200 sample injections during method development. The ovomucoid column showed generally higher resolution, greater flexibility in operating parameters, and better long-term stability than the acid glycoprotein column.