THE ISOLATION AND IDENTIFICATION OF 6-HYDROXYCYCLOHEPTA-1,4-DIONE AS A NOVEL INTERMEDIATE IN THE BACTERIAL-DEGRADATION OF ATROPINE

被引：11

作者：

BARTHOLOMEW, BA ^{[1
]}

SMITH, MJ ^{[1
]}

LONG, MT ^{[1
]}

DARCY, PJ ^{[1
]}

TRUDGILL, PW ^{[1
]}

HOPPER, DJ ^{[1
]}

机构：

[1] UNIV WALES,DEPT BIOCHEM,ABERYSTWYTH SY23 3DD,DYFED,WALES

来源：

BIOCHEMICAL JOURNAL | 1993年 / 293卷

关键词：

D O I：

10.1042/bj2930115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Growth of Pseudomonas AT3 on the alkaloid atropine as its sole source of carbon and nitrogen is nitrogen-limited and proceeds by degradation of the tropic acid part of the molecule, with the metabolism of the tropine being limited to the point of release of its nitrogen. A nitrogen-free compound accumulated in the growth medium and was isolated and identified as 6-hydroxy-cyclohepta-1,4-dione, This novel compound is proposed as an intermediate in tropine metabolism. It served as a growth substrate for the organism and was also the substrate for an NAD+-linked dehydrogenase present in cell extracts. The enzyme was induced during the tropine phase of diauxic growth on atropine or during growth on tropine alone.

引用

页码：115 / 118

页数：4