SEQUENCE AND CHARACTERIZATION OF THE MET-7 GENE OF NEUROSPORA-CRASSA

被引:5
作者
CRAWFORD, JM [1 ]
GEEVER, RF [1 ]
ASCH, DK [1 ]
CASE, ME [1 ]
机构
[1] UNIV GEORGIA,DEPT GENET,C314 LIFE SCI BLDG,ATHENS,GA 30602
关键词
RECOMBINANT DNA; FUNGAL BIOCHEMISTRY; METHIONINE BIOSYNTHETIC PATHWAY; CYSTATHIONINE-GAMMA-SYNTHASE;
D O I
10.1016/0378-1119(92)90698-O
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino acid open reading frame (ORF). Disruption of this ORF abolishes complementation of the met-7 mutation.
引用
收藏
页码:265 / 266
页数:2
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