HIGH-EFFICIENCY TRANSFORMATION AND GENE INACTIVATION IN STREPTOCOCCUS-SUIS TYPE-2

被引：55

作者：

SMITH, HE ^{[1
]}

WISSELINK, HJ ^{[1
]}

VECHT, U ^{[1
]}

GIELKENS, ALJ ^{[1
]}

SMITS, MA ^{[1
]}

机构：

[1] INST ANIM SCI & HLTH,DEPT BACTERIOL,8200 AB LELYSTAD,NETHERLANDS

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

TRANSFORMATION; HOMOLOGOUS RECOMBINATION; DOUBLE AND SINGLE CROSS-OVER INTEGRATIONS; GENE AMPLIFICATION;

D O I：

10.1099/00221287-141-1-181

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An efficient electrotransformation system for Streptococcus suis type 2 is described. It is demonstrated that vectors based on the broad-host-range plasmid pWVO1 replicate in S. suis type 2. Transformation efficiencies of about 10(7) transformants per mu g of plasmid DNA could be obtained, Derivatives of plasmid pBR322 containing S. suis chromosomal DNA did not replicate but integrated into the chromosome. Southern hybridization analysis revealed that double as well as single cross-over integration events had occurred. Double cross-over events occurred at a frequency of about 15%. With these transformation and integration systems. recombinant DNA technology can now be applied to this important pathogenic species.

引用

页码：181 / 188

页数：8

共 32 条

[1]

ARENDS JP, 1988, REV INFECT DIS, V10, P131

[2]

BRON S, 1990, MOL BIOL METHODS BAC, P75

[3]

CLIFTONHADLEY FA, 1983, BRIT VET J, V139, P1

[4] HIGH-EFFICIENCY TRANSFORMATION OF ESCHERICHIA-COLI BY HIGH-VOLTAGE ELECTROPORATION [J].

DOWER, WJ ;

MILLER, JF ;

RAGSDALE, CW .

NUCLEIC ACIDS RESEARCH, 1988, 16 (13) :6127-6145

[5] IMPROVED ELECTROPORATION AND CLONING VECTOR SYSTEM FOR GRAM-POSITIVE BACTERIA [J].

DUNNY, GM ;

LEE, LN ;

LEBLANC, DJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (04) :1194-1201

[6] HIGH-FREQUENCY TRANSFORMATION, BY ELECTROPORATION, OF LACTOCOCCUS-LACTIS SUBSP CREMORIS GROWN WITH GLYCINE IN OSMOTICALLY STABILIZED MEDIA [J].

HOLO, H ;

NES, IF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (12) :3119-3123

[7] NUCLEOTIDE-SEQUENCE AND FUNCTIONAL MAP OF PC194, A PLASMID THAT SPECIFIES INDUCIBLE CHLORAMPHENICOL RESISTANCE [J].

HORINOUCHI, S ;

WEISBLUM, B .

JOURNAL OF BACTERIOLOGY, 1982, 150 (02) :815-825

[8] STABLE GENE AMPLIFICATION IN THE CHROMOSOME OF BACILLUS-SUBTILIS [J].

JANNIERE, L ;

NIAUDET, B ;

PIERRE, E ;

EHRLICH, SD .

GENE, 1985, 40 (01) :47-55

[9]

KOK J, 1991, GENETICS AND MOLECULAR BIOLOGY OF STREPTOCOCCI, LACTOCOCCI, AND ENTEROCOCCI, P97

[10] CONSTRUCTION OF PLASMID CLONING VECTORS FOR LACTIC STREPTOCOCCI WHICH ALSO REPLICATE IN BACILLUS-SUBTILIS AND ESCHERICHIA-COLI [J].

KOK, J ;

VANDERVOSSEN, JMBM ;

VENEMA, G .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (04) :726-731

← 1 2 3 4 →