A PHOSPHOLIPID-DEPENDENT NADH-COENZYME-Q REDUCTASE FROM LIVER PLASMA-MEMBRANE

被引：74

作者：

NAVARRO, F

VILLALBA, JM

CRANE, FL

MACKELLAR, WC

NAVAS, P

机构：

[1] UNIV CORDOBA,FAC CIENCIAS,DEPT BIOL CELULAR,E-14004 CORDOBA,SPAIN

[2] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907

[3] ELI LILLY & CO,LILLY CORP CTR,LILLY RES LABS,INDIANAPOLIS,IN 46285

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 212卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.1947

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 34 kDa coenzyme Q reductase has been solubilized and purified from pig liver plasma membranes. The solubilized enzyme reduced coenzyme Q(0) with NADH. Ubiquinones with longer isoprenoid side chain such as Q(2) and Q(10) were also reduced when the quinones and the enzyme were reconstituted into phospholipid liposomes. N-terminal sequencing of an internal peptide showed identity to bovine NADH-cytochrome b(5) reductase. Biochemical characterization of the purified enzyme indicated that the coenzyme Q reductase corresponds to an unusual form of NADH-cytochrome bg reductase. (C) 1995 Academic Press, Inc.

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页码：138 / 143

页数：6

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