RECONSTITUTION OF SIMIAN VIRUS-40 DNA-REPLICATION WITH PURIFIED PROTEINS

被引：142

作者：

WEINBERG, DH ^{[1
]}

COLLINS, KL ^{[1
]}

SIMANCEK, P ^{[1
]}

RUSSO, A ^{[1
]}

WOLD, MS ^{[1
]}

VIRSHUP, DM ^{[1
]}

KELLY, TJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 22期

关键词：

DNA polymerase δ; eukaryotic DNA replication; replication factor C;

D O I：

10.1073/pnas.87.22.8692

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Replication of plasmid DNA molecules containing the simian virus 40 (SV40) origin of DNA replication has been reconstituted with seven highly purified cellular proteins plus the SV40 large tumor (T) antigen. Initiation of DNA synthesis is absolutely dependent upon T antigen, replication protein A, and the DNA polymerase α-primase complex and is stimulated by the catalytic subunit of protein phosphatase 2A. Efficient elongation of nascent chains additionally requires proliferating cell nuclear antigen, replication factor C, DNA topoisomerase I, and DNA polymerase δ. Electron microscopic studies indicate that DNA replication begins at the viral origin and proceeds via intermediates containing two forks that move in opposite directions. These findings indicate that the reconstituted replication reaction has many of the characteristics expected of authentic viral DNA replication.

引用

页码：8692 / 8696

页数：5

共 37 条

[1] LOCALIZED MELTING AND STRUCTURAL-CHANGES IN THE SV40 ORIGIN OF REPLICATION INDUCED BY T-ANTIGEN [J].

BOROWIEC, JA ;

HURWITZ, J .

EMBO JOURNAL, 1988, 7 (10) :3149-3158

[2] ATP STIMULATES THE BINDING OF SIMIAN VIRUS-40 (SV40) LARGE TUMOR-ANTIGEN TO THE SV40 ORIGIN OF REPLICATION [J].

BOROWIEC, JA ;

HURWITZ, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :64-68

[3]

CHALLBERG MD, 1989, ANNU REV BIOCHEM, V58, P671

[4]

DAVIS RW, 1971, METHOD ENZYMOL, V21, P413

[5] SIMIAN-VIRUS 40 (SV40) DNA-REPLICATION - SV40 LARGE T-ANTIGEN UNWINDS DNA CONTAINING THE SV40 ORIGIN OF REPLICATION [J].

DEAN, FB ;

BULLOCK, P ;

MURAKAMI, Y ;

WOBBE, CR ;

WEISSBACH, L ;

HURWITZ, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (01) :16-20

[6] ATP ENHANCES THE BINDING OF SIMIAN VIRUS-40 LARGE T-ANTIGEN TO THE ORIGIN OF REPLICATION [J].

DEB, SP ;

TEGTMEYER, P .

JOURNAL OF VIROLOGY, 1987, 61 (12) :3649-3654

[7]

DOWNEY KM, 1988, CANCER CELLS EUKARYO, V6, P403

[8] CELLULAR FACTORS REQUIRED FOR MULTIPLE STAGES OF SV40 DNA-REPLICATION INVITRO [J].

FAIRMAN, MP ;

STILLMAN, B .

EMBO JOURNAL, 1988, 7 (04) :1211-1218

[9]

GOETZ GS, 1988, J BIOL CHEM, V263, P383

[10]

ISHIMI Y, 1988, J BIOL CHEM, V263, P19723

← 1 2 3 4 →