PRIMARY STRUCTURE OF RAT RENAL DIPEPTIDASE AND EXPRESSION OF ITS MESSENGER-RNA IN RAT-TISSUES AND COS-1 CELLS

被引：18

作者：

ADACHI, H

ISHIDA, N

TSUJIMOTO, M

机构：

[1] Suntory Institute for Biomedical Research, Mishima-gun, Osaka, Shimamoto-cho

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 03期

关键词：

RENAL DIPEPTIDASE; CDNA; GENE EXPRESSION; COS-1; CELL;

D O I：

10.1016/0167-4781(92)90167-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three cDNA clones of rat renal dipeptidase (rrDP) were isolated from rat renal and pulmonary cDNA libraries using a DNA fragment of human renal DP cDNA clone, MDP4, as a probe. The complete amino acid sequence deduced from the cDNA contains 410 amino acid residues, beginning with a signal peptide of 16 amino acid residues. RNA blot hybridization analysis showed that 1.6 and 2.2 kb mRNAs were expressed in lung and kidney, however, only 1.6 kb mRNA was detected in small intestine. COS-1 cells transfected with the cDNA expressed enzymatically active rrDP.

引用

页码：311 / 314

页数：4

共 12 条

[1]

ADACHI H, 1990, J BIOL CHEM, V265, P15341

[2] PURIFICATION AND CHARACTERIZATION OF HUMAN MICROSOMAL DIPEPTIDASE [J].

ADACHI, H ;

KUBOTA, I ;

OKAMURA, N ;

IWATA, H ;

TSUJIMOTO, M ;

NAKAZATO, H ;

NISHIHARA, T ;

NOGUCHI, T .

JOURNAL OF BIOCHEMISTRY, 1989, 105 (06) :957-961

[3]

ADACHI H, 1990, J BIOL CHEM, V265, P3992

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P136

[5] CHARACTERIZATION OF DEHYDROPEPTIDASE-I IN THE RAT LUNG [J].

HIROTA, T ;

NISHIKAWA, Y ;

TANAKA, M ;

IGARASHI, T ;

KITAGAWA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 160 (03) :521-525

[6] CLONING OF CDNAS ENCODING A RABBIT RENAL BRUSH-BORDER MEMBRANE-PROTEIN IMMUNOLOGICALLY RELATED TO BAND-3 - SEQUENCE SIMILARITY WITH MICROSOMAL DIPEPTIDASE [J].

IGARASHI, P ;

KARNISKI, LP .

BIOCHEMICAL JOURNAL, 1991, 280 :71-78

[7]

KOZAK EM, 1982, J BIOL CHEM, V257, P6322

[8] METABOLISM OF THIENAMYCIN AND RELATED CARBAPENEM ANTIBIOTICS BY THE RENAL DIPEPTIDASE, DEHYDROPEPTIDASE-I [J].

KROPP, H ;

SUNDELOF, JG ;

HAJDU, R ;

KAHAN, FM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1982, 22 (01) :62-70

[9]

KURIBAYASHI K, 1988, NUCLEIC ACIDS RES S, V19, P61

[10]

MANIATIS T, 1982, MOL CLONING

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