HAEMONCHUS-CONTORTUS - IVERMECTIN-INDUCED PARALYSIS OF THE PHARYNX

被引:187
作者
GEARY, TG
SIMS, SM
THOMAS, EM
VANOVER, L
DAVIS, JP
WINTERROWD, CA
KLEIN, RD
HO, NFH
THOMPSON, DP
机构
[1] UPJOHN CO,UPJOHN LABS,DRUG DELIVERY SYST RES,KALAMAZOO,MI 49001
[2] UPJOHN CO,UPJOHN LABS,MOLEC BIOL RES,KALAMAZOO,MI 49001
关键词
HAEMONCHUS-CONTORTUS; NEMATODE; PHARYNX; PARASITE; IVERMECTIN (IVM); ADENOSINE TRIPHOSPHATE (ATP); GAMMA-AMINOBUTYRIC ACID (GABA); DIMETHYL SULFOXIDE (DMSO); FLUORESCEIN ISOTHIOCYANATE (FITC); CARBONYLCYANIDE M-CHLOROPHENYLHYDRAZONE (CCCP); ADENOSINE MONOPHOSPHATE (AMP); 3-TRIMETHYL PROPANE SULFONIC ACID; NA+; SALT; (DSS);
D O I
10.1006/expr.1993.1064
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations ≥10-10M. Motility, monitored quantitatively with an automated motility meter, was unaffected by IVM at concentrations ≤10-8M, while ATP levels were unaffected at concentrations ≤10-6M. Since motility and ATP levels, independent measures of short-term viability, are unaffected by concentrations of IVM that effectively block oral ingestion, the drug can be used as a chemical ligature. Although H. contortus was shown to be dependent upon an exogenous supply of glucose for survival in culture, IVM (l0-9 and 10-7M) altered neither the uptake of 3-O-[3H]methylglucose nor the metabolism of [13C]glucose by the parasite. These data suggest that H. contortus depends upon the transcuticular uptake of glucose in culture. If oral ingestion of other nutrients is essential for long-term survival in vivo, disruption of this process may represent the primary mechanism of IVM action. © 1993 Academic press, Inc.
引用
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页码:88 / 96
页数:9
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