B-CELLS REGULATE EXPRESSION OF CD40 LIGAND ON ACTIVATED T-CELLS BY LOWERING THE MESSENGER-RNA LEVEL AND THROUGH THE RELEASE OF SOLUBLE CD40

被引:150
作者
VANKOOTEN, C
GAILLARD, C
GALIZZI, JP
HERMANN, P
FOSSIEZ, F
BANCHEREAU, J
BLANCHARD, D
机构
[1] Schering-Plough, Laboratory for Immunological Research, Dardilly
关键词
CD40; LIGAND; T CELL; B CELL;
D O I
10.1002/eji.1830240402
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The expression of CD40 ligand (CD40L) on activated T cells (CD4(+) T cell clone MT9) is diminished when the T cells are cultured in the presence of B cells. This effect, observed both with normal tonsil B cells and with the B cell line JY, was detected after 6 h and sustained at least until 18 h of co-culture. Analysis of mRNA showed that CD40L mRNA levels were not modified after 6 h, but were significantly down-regulated after 18 h of co-culture with B cells. Although CD40L expression could not be detected by a CD40-Fc chimera, the molecule was still expressed at the membrane as shown with a polyclonal antiserum against CD40L (anti-TRAP). In addition, T cells activated in the presence of B cells were stained by a polyclonal antiserum against CD40, without the appearance of CD40 mRNA. These results indicated that a soluble form of CD40 (sCD40) bound to the expressed CD40L on T cells. The existence of sCD40 was confirmed by detection of sCD40 in B cell supernatants using a specific enzyme-linked immunosorbent assay. Collectively, these data show that B cells can regulate the expression of CD40L on activated T cells at least by two different mechanisms.
引用
收藏
页码:787 / 792
页数:6
相关论文
共 47 条
[1]   CD40 LIGAND GENE DEFECTS RESPONSIBLE FOR X-LINKED HYPER-IGM SYNDROME [J].
ALLEN, RC ;
ARMITAGE, RJ ;
CONLEY, ME ;
ROSENBLATT, H ;
JENKINS, NA ;
COPELAND, NG ;
BEDELL, MA ;
EDELHOFF, S ;
DISTECHE, CM ;
SIMONEAUX, DK ;
FANSLOW, WC ;
BELMONT, J ;
SPRIGGS, MK .
SCIENCE, 1993, 259 (5097) :990-993
[2]   IDENTIFICATION OF A SOURCE OF BIOLOGICALLY-ACTIVE CD40 LIGAND [J].
ARMITAGE, RJ ;
SATO, TA ;
MACDUFF, BM ;
CLIFFORD, KN ;
ALPERT, AR ;
SMITH, CA ;
FANSLOW, WC .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (08) :2071-2076
[3]   MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF A MURINE LIGAND FOR CD40 [J].
ARMITAGE, RJ ;
FANSLOW, WC ;
STROCKBINE, L ;
SATO, TA ;
CLIFFORD, KN ;
MACDUFF, BM ;
ANDERSON, DM ;
GIMPEL, SD ;
DAVISSMITH, T ;
MALISZEWSKI, CR ;
CLARK, EA ;
SMITH, CA ;
GRABSTEIN, KH ;
COSMAN, D ;
SPRIGGS, MK .
NATURE, 1992, 357 (6373) :80-82
[4]   CD40 LIGAND IS A T-CELL GROWTH-FACTOR [J].
ARMITAGE, RJ ;
TOUGH, TW ;
MACDUFF, BM ;
FANSLOW, WC ;
SPRIGGS, MK ;
RAMSDELL, F ;
ALDERSON, MR .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (09) :2326-2331
[5]   THE CD40 LIGAND, GP39, IS DEFECTIVE IN ACTIVATED T-CELLS FROM PATIENTS WITH X-LINKED HYPER-IGM SYNDROME [J].
ARUFFO, A ;
FARRINGTON, M ;
HOLLENBAUGH, D ;
LI, X ;
MILATOVICH, A ;
NONOYAMA, S ;
BAJORATH, J ;
GROSMAIRE, LS ;
STENKAMP, R ;
NEUBAUER, M ;
ROBERTS, RL ;
NOELLE, RJ ;
LEDBETTER, JA ;
FRANCKE, U ;
OCHS, HD .
CELL, 1993, 72 (02) :291-300
[6]   HUMAN-B LYMPHOCYTES - PHENOTYPE, PROLIFERATION, AND DIFFERENTIATION [J].
BANCHEREAU, J ;
ROUSSET, F .
ADVANCES IN IMMUNOLOGY, 1992, 52 :125-262
[7]   LONG-TERM HUMAN B-CELL LINES DEPENDENT ON INTERLEUKIN-4 AND ANTIBODY TO CD40 [J].
BANCHEREAU, J ;
DEPAOLI, P ;
VALLE, A ;
GARCIA, E ;
ROUSSET, F .
SCIENCE, 1991, 251 (4989) :70-72
[8]   ROLE OF CD40 ANTIGEN AND INTERLEUKIN-2 IN T-CELL-DEPENDENT HUMAN B-LYMPHOCYTE GROWTH [J].
BLANCHARD, D ;
GAILLARD, C ;
HERMANN, P ;
BANCHEREAU, J .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1994, 24 (02) :330-335
[9]  
CASTLE BE, 1993, J IMMUNOL, V151, P1777
[10]   GM-CSF AND TNF-ALPHA COOPERATE IN THE GENERATION OF DENDRITIC LANGERHANS CELLS [J].
CAUX, C ;
DEZUTTERDAMBUYANT, C ;
SCHMITT, D ;
BANCHEREAU, J .
NATURE, 1992, 360 (6401) :258-261