AUTOMATED SCREENING SYSTEM FOR PURINE AND PYRIMIDINE METABOLISM DISORDERS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：50

作者：

SUMI, S

KIDOUCHI, K

OHBA, S

WADA, Y

机构：

[1] NAGOYA CITY HIGASHI GEN HOSP,DEPT PEDIAT,CHIKUSA KU,NAGOYA,AICHI 464,JAPAN

[2] NAGOYA CITY UNIV,SCH MED,DEPT PEDIAT,MIZUHO KU,NAGOYA,AICHI 467,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 672卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00228-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated screening system for purine and pyrimidine metabolism disorders using high-performance liquid chromatography (HPLC) with column switching is described. The system consists of a reversed-phase column, a cation-exchange column, a column switch, four sets of ultraviolet absorbance detectors, a microcomputer and other conventional equipment. As this system permits the simultaneous determination of urinary erotic acid, uracil, dihydrouracil, pseudouridine, xanthine, 2,8-dihydroxyadenine and succinyladenosine, it offers a useful method for the detection of erotic aciduria, dihydropyrimidine dehydrogenase deficiency, diphydropyrimidinuria, xanthinuria, adenine phosphoribosyltransferase deficiency and adenylosuccinase deficiency.

引用

页码：233 / 239

页数：7

共 12 条

[1] DIAGNOSIS OF INHERITED ADENYLOSUCCINASE DEFICIENCY BY THIN-LAYER CHROMATOGRAPHY OF URINARY IMIDAZOLES AND BY AUTOMATED CATION-EXCHANGE COLUMN CHROMATOGRAPHY OF PURINES [J].