STRATEGIES FOR OBTAINING STABLE ENZYMES

被引：53

作者：

JANECEK, S

机构：

[1] Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, 81237 Bratislava, Radlinského 9, SQ

来源：

PROCESS BIOCHEMISTRY | 1993年 / 28卷 / 07期

关键词：

D O I：

10.1016/0032-9592(93)85026-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Generally, enzymes are only marginally stable. In order to increase their utilization as industrial biocatalysts, it is necessary to obtain enzymes with enhanced operational as well as survival stability. Two principal ways exist to fulfil this task. (i) the use of enzymes which possess natural extremostability, or (ii) stabilization of naturally unstable enzymes. These are reviewed separately in order to describe the individual approaches, mainly immobilization, chemical modification and protein engineering. Some prospective trends (solvent engineering and protein design) are suggested as being potentially useful to provide a wider insight into enzymes designed for industrial processes.

引用

页码：435 / 445

页数：11

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