CLONING OF 2 ISOFORMS OF THE RAT-BRAIN NA+-CA-2+ EXCHANGER GENE AND THEIR FUNCTIONAL EXPRESSION IN HELA-CELLS

被引：83

作者：

FURMAN, I ^{[1
]}

COOK, O ^{[1
]}

KASIR, J ^{[1
]}

RAHAMIMOFF, H ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT BIOCHEM,POB 1172,IL-91010 JERUSALEM,ISRAEL

来源：

FEBS LETTERS | 1993年 / 319卷 / 1-2期

关键词：

NA+-CA-2+ EXCHANGE; CALCIUM REGULATION; BRAIN; CLONING; EXPRESSION; HELA CELL;

D O I：

10.1016/0014-5793(93)80046-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two functional isoforms of the rat brain Na+ Ca2+ exchanger were isolated from a lambdaZAP hippocampus cDNA library. The open reading frame of clone RBE-1 codes for a protein 935 amino acids long, and that of clone RBE-2 codes for a protein 958 amino acids long. Expression in HeLa cells of Na+ gradient dependent Ca2+ transport activity was determined following transfection of the cells with either RBE-1 or RBE-2. Both clones expressed proteins that exchange Na+ with Ca2+ in an electrogenic manner and none of them exhibited a dependency of the antiport on K+, since they transported Ca2+ in an Na+ gradient dependent manner in external choline chloride as well.

引用

页码：105 / 109

页数：5

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