ISOLATION AND CHARACTERIZATION OF COAGGREGATION-DEFECTIVE (COG(-)) MUTANTS OF STREPTOCOCCUS-GORDONII DL1 (CHALLIS)

被引：9

作者：

CLEMANS, DL ^{[1
]}

KOLENBRANDER, PE ^{[1
]}

机构：

[1] NIDR,MICROBIAL ECOL LAB,BETHESDA,MD 20892

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1995年 / 15卷 / 03期

关键词：

COAGGREGATION-DEFECTIVE MUTANTS; ACCRETION; ORAL STREPTOCOCCI; ORAL BIOFILMS; ADHESINS;

D O I：

10.1007/BF01569825

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Streptococcus gordonii DL1 (Challis) bears coaggregation-mediating surface adhesins which recognize galactoside-containing surface polysaccharides on Streptococcus oralis 34, Streptococcus oralis C104, and Streptococcus SM PK509. Fifty-nine spontaneously-occurring coaggregation-defective (Cog(-)) mutants of S. gordonii DL1 unable to coaggregate with partner streptococci were isolated. Six representative Cog(-) mutants were characterized by their coaggregation properties with four Actinomyces naeslundii strains (T14V, PK947, PK606, PK984), Veillonella atypica PK1910, and Propionibacterium acnes PK93. The six representative Cog(-) mutants showed altered coaggregation with their streptococcal partners, A. naeslundii PK947, and P. acnes PK93. Based on the coaggregation phenotypes of these mutants, a model for the lactose-inhibitable coaggregation between S. gordonii DL1 and its partner bacteria is proposed. The potential use of these mutants in studies of oral biofilms is discussed.

引用

页码：193 / 197

页数：5

共 20 条

[1] COMPLETE STRUCTURE OF THE CELL-SURFACE POLYSACCHARIDE OF STREPTOCOCCUS-ORALIS-C104 - A 600-MHZ NMR-STUDY [J].