PHENYTOIN FREE FRACTION DETERMINATION - COMPARISON OF AN IMPROVED DIRECT SERUM INJECTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD TO ULTRAFILTRATION COUPLED WITH FLUORESCENCE POLARIZATION IMMUNOASSAY

被引：24

作者：

GURLEY, BJ

MARX, M

OLSEN, K

机构：

[1] UNIV ARKANSAS MED SCI HOSP,COLL PHARM,DEPT PHARM PRACTICE,LITTLE ROCK,AR 72205

[2] UNIV NEBRASKA,MED CTR,COLL PHARM,DEPT PHARM PRACTICE,OMAHA,NE 68198

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 670卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00171-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in restricted-access media (RAM) liquid chromatography make the simultaneous determination of total and free phenytoin concentrations possible by direct injection of drug-containing serum samples, A comparison of phenytoin free fraction determination by ultrafiltration coupled with fluorescence polarization immunoassay (TDX) to an improved direct injection RAM-HPLC method is presented. Our improved method differs from those previously reported with regard to column type, mobile-phase composition, and column temperature. Replicate samples analyzed by each method yielded similar values for serum phenytoin free fraction.

引用

页码：358 / 364

页数：7

共 22 条

[1] USE OF PLASMA UNBOUND DRUG CONCENTRATIONS IN ADJUSTING PHENYTOIN DOSES [J].