A NEUTRALIZING MONOCLONAL-ANTIBODY AGAINST COXSACKIEVIRUS-B4 CROSS-REACTS WITH CONTRACTILE MUSCLE PROTEINS

被引：15

作者：

BEISEL, KW ^{[1
]}

SRINIVASAPPA, J ^{[1
]}

OLSEN, MR ^{[1
]}

STIFF, AC ^{[1
]}

ESSANI, K ^{[1
]}

PRABHAKAR, BS ^{[1
]}

机构：

[1] NIDR,ORAL MED LAB,BETHESDA,MD 20892

来源：

MICROBIAL PATHOGENESIS | 1990年 / 8卷 / 02期

关键词：

Coxsackievirus B4; heart muscle; molecular mimicry; monoclonal antibodies;

D O I：

10.1016/0882-4010(90)90079-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A panel of Coxsackievirus B4 (CVB4) neutralizing monoclonal antibodies (mAbs) were tested against a panel of normal mouse tissues. One mAb, 356-1, reacted specifically with murine heart tissue. Immunohistochemical studies revealed an A band pattern of staining of the heart. Examination of sequential differential extracts of heart by Western immunoblotting showed that 356-1 predominantly reacted with the murine cardiac myosin heavy chain. A rather weak cross-reaction was found with actin. These observations were confirmed by the binding of 356-1 to purified cardiac myosin and actin. This antibody showed a higher affinity for murine cardiac muscle myosin than for skeletal muscle myosin. Examination of the reactivity of 356-1 with CVB4 polypeptides using Western immunoblotting revealed that 356-1 binds to the VP-1 capsid protein. These studies imply that molecular mimicry is one mechanism by which autoimmunity could develop during CVB4 induced myocarditis. © 1990.

引用

页码：151 / 156

页数：6

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