MOLECULAR-CLONING OF SUBSTANCE-P RECEPTOR CDNA FROM GUINEA-PIG UTERUS

被引：35

作者：

GORBULEV, V ^{[1
]}

AKHUNDOVA, A ^{[1
]}

LUZIUS, H ^{[1
]}

FAHRENHOLZ, F ^{[1
]}

机构：

[1] MAX PLANCK INST BIOPHYS,KENNEDY ALLEE 70,W-6000 FRANKFURT 70,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1131卷 / 01期

关键词：

SUBSTANCE-P RECEPTOR; G-PROTEIN-COUPLED RECEPTOR; GUINEA-PIG UTERUS; COS CELL EXPRESSION;

D O I：

10.1016/0167-4781(92)90105-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding guinea-pig uterine substance P (SP) receptor has been isolated using the homology screening approach. Northern blot analysis reveals that the corresponding mRNA, of approx. 4.8 kb, is expressed in all tissues tested, but predominantly in the uteri of non-pregnant animals; during pregnancy its expression is reduced. The guinea-pig SP receptor was expressed in COS-7 cells and demonstrated relative ligand affinity in the order: SP much greater than neurokinin A > neurokinin B.

引用

页码：99 / 102

页数：4

共 16 条

[1] ALM P, 1988, CELL TISSUE RES, V254, P517
[2] TRANSCRIPTION TERMINATION AND 3' PROCESSING - THE END IS IN SITE
BIRNSTIEL, ML
BUSSLINGER, M
STRUB, K
[J]. CELL, 1985, 41 (02) : 349 - 359
[3] SELECTIVE AGONISTS FOR SUBSTANCE-P AND NEUROKININ RECEPTORS
DRAPEAU, G
DORLEANSJUSTE, P
DION, S
RHALEB, NE
ROUISSI, NE
REGOLI, D
[J]. NEUROPEPTIDES, 1987, 10 (01) : 43 - 54
[4] GAMMELTOFT S, 1990, PEPTIDE HORMONE ACTI, P1
[5] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING
HENIKOFF, S
[J]. GENE, 1984, 28 (03) : 351 - 359
[6] MOLECULAR CHARACTERIZATION OF A FUNCTIONAL CDNA-ENCODING THE RAT SUBSTANCE-P RECEPTOR
HERSHEY, AD
KRAUSE, JE
[J]. SCIENCE, 1990, 247 (4945) : 958 - 962
[7] LOEUILLET D, 1989, INT J PEPT PROT RES, V33, P171
[8] HIGH-LEVEL TRANSIENT EXPRESSION OF A CHLORAMPHENICOL ACETYL TRANSFERASE GENE BY DEAE-DEXTRAN MEDIATED DNA TRANSFECTION COUPLED WITH A DIMETHYLSULFOXIDE OR GLYCEROL SHOCK-TREATMENT
LOPATA, MA
CLEVELAND, DW
SOLLNERWEBB, B
[J]. NUCLEIC ACIDS RESEARCH, 1984, 12 (14) : 5707 - 5717
[9] PREGNANCY REDUCES NORADRENALINE BUT NOT NEUROPEPTIDE LEVELS IN THE UTERINE ARTERY OF THE GUINEA-PIG
MIONE, MC
CAVANAGH, JFR
LINCOLN, J
MILNER, P
BURNSTOCK, G
[J]. CELL AND TISSUE RESEARCH, 1990, 259 (03) : 503 - 509
[10] MUNSON PJ, 1983, METHOD ENZYMOL, V92, P543

← 1 2 →