EVALUATION OF RADIOACTIVE AND NONRADIOACTIVE GENE PROBES AND CELL-CULTURE FOR DETECTION OF POLIOVIRUS IN WATER SAMPLES

被引：4

作者：

MOORE, NJ

MARGOLIN, AB

机构：

[1] UNIV NEW HAMPSHIRE,MOLEC PATHOGENESIS & INFECT DIS GRP,DURHAM,NH 03824

[2] UNIV NEW HAMPSHIRE,SPAULDING LIFE SCI CTR,DURHAM,NH 03824

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 09期

关键词：

D O I：

10.1128/AEM.59.9.3145-3146.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Five nonradioactive probe assays were evaluated by using chemiluminescent and colormetric signals, along with two isotopic assays and cell culture, for the detection of poliovirus in concentrated water samples. In environmental samples, a 100% correlation existed between digoxigenin and single-stranded [P-32]RNA probes. All probe assays detected more positive samples than the cell culture did.

引用

页码：3145 / 3146

页数：2

共 10 条

[1] ROTAVIRUS IN TRAVELERS DIARRHEA - STUDY OF AN ADULT STUDENT POPULATION IN MEXICO [J].