INTERACTION OF EXCISION-REPAIR GENE-PRODUCTS AND MITOTIC RECOMBINATION FUNCTIONS IN YEAST

被引:6
作者
MONTELONE, BA [1 ]
LIANGCHONG, BC [1 ]
机构
[1] KANSAS STATE UNIV AGR & APPL SCI,DEPT PHYS,MANHATTAN,KS 66506
关键词
MITOTIC RECOMBINATION; RAD3; GENE; NUCLEOTIDE EXCISION REPAIR; YEAST;
D O I
10.1007/BF00351709
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We have tested the ability of mutants of three additional genes in the excision repair pathway of Saccharomyces cerevisiae to suppress the hyper-recombination and rad52 double-mutant lethality phenotypes of the rad3-102 (formerly rem1-2) mutation. Such suppression has previously been been observed with mutant alleles of RAD1 and RAD4. We had hypothesized that the rad3-102 mutation created elevated levels of DNA lesions which could be processed by the products of the RAD1 and RAD4 genes into recombinogenic double-strand breaks requiring the RAD52 product for repair. In this report, we show that the RAD2, RAD7, and RAD10 genes are also necessary for this processing. We discuss our observations of varying levels of mitotic crossing-over in Rem- rad double-mutant strains.
引用
收藏
页码:481 / 486
页数:6
相关论文
共 39 条
[1]  
Adzuma K., Ogawa T., Ogawa H., Mol Cell Biol, 4, pp. 2735-2744, (1984)
[2]  
Bailis A.M., Rothstein R., Genetics, 126, pp. 535-547, (1990)
[3]  
Bailis A.M., Arthur L., Rothstein R., Mol Cell Biol, 12, pp. 4988-4993, (1992)
[4]  
Bardwell L., Cooper A.J., Friedberg E.C., Mol Cell Biol, 12, pp. 3041-3049, (1992)
[5]  
von Borstel R.C., Cain K.T., Steinberg C.M., Genetics, 69, pp. 17-27, (1971)
[6]  
Fishman-Lobell J., Haber J.E., Science, 258, pp. 480-484, (1992)
[7]  
Friedberg E.C., Mol Microbiol, 5, pp. 2303-2310, (1991)
[8]  
Golin J.E., Esposito, Mol Gen Genet, 150, pp. 127-135, (1977)
[9]  
Harosh I., Naumovski L., Friedberg E.C., J Biol Chem, 264, pp. 20532-20539, (1989)
[10]  
Hastings P.J., Quah S.K., von Borstel R.C., Nature, 264, pp. 719-722, (1976)