CDNA SEQUENCE-ANALYSIS OF THE HUMAN BRAIN INSULIN-RECEPTOR

被引：16

作者：

KENNER, KA

KUSARI, J

HEIDENREICH, KA

机构：

[1] UNIV COLORADO,HLTH SCI CTR,DEPT PHARMACOL C236,DENVER,CO 80262

[2] UNIV CALIF SAN DIEGO,DEPT MED,DIV ENDOCRINOL & METAB,LA JOLLA,CA 92093

[3] VET ADM MED CTR,MED RES SERV,SAN DIEGO,CA 92161

[4] TULANE UNIV,MED CTR,SCH MED,DEPT PHYSIOL,NEW ORLEANS,LA 70112

[5] VET AFFAIRS MED RES CTR,DENVER,CO 80220

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 217卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2778

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Brain tissue mRNA was amplified using polymerase chain reaction (PCR) with eight overlapping sets of primers that span the cDNA coding sequence for the human placental insulin receptor. Only the A isoform (Irking exon 11) of the receptor was detected. No difference was found in the predicted amino acid sequence of brain derived insulin receptor cDNA compared with the receptor from human placenta. A silent polymorphism was detected at nucleotide position 1698 (amino acid 523), confirming that mRNA corresponding to both alleles of the human brain receptor was sequenced. Our findings indicate that the unique glycosylation properties of brain insulin receptors do not stem from differences in primary structure, but rather are due to tissue-specific differences in post-translational processing. (C) 1995 Academic Press. Inc.

引用

页码：304 / 312

页数：9

共 33 条

[1] INTERNALIZATION AND MOLECULAR PROCESSING OF INSULIN-RECEPTORS IN ISOLATED RAT ADIPOCYTES [J].