Comparison of solvent modulation with premixed mobile phases for the separation of corticosteroids by liquid chromatography

被引：6

作者：

Lukulay, PH

McGuffin, VL

机构：

[1] Department of Chemistry, Michigan State University, East Lansing, Michigan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 20期

关键词：

D O I：

10.1080/10826079508013744

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The separation of eight common corticosteroids is optimized by solvent modulation and by conventional premixed solvents. The separation is achieved on an octadecylsilica column using aqueous acetonitrile and methanol mobile phases. The optimized separations are compared with respect to accuracy, total analysis time, critical resolution, and overall quality of the separation. The solvent modulation approach compares favorably to premixed solvents in all of these respects and, hence, is demonstrated to be a very promising optimization strategy.

引用

页码：4039 / 4062

页数：24

共 40 条

[1]

Hildebrand J.H., Scott R.L., (1950)

[2]

F. M. Barton, Chem A., 75, (1975)

[3]

D. E. Martire, D. C. Locke, Anal. Chem., 43:68 (1971)

[4]

Schoenmakers P.J., Billiet H.A.H., de Galan L., Chromatogr J., 179, (1979)

[5]

Schoenmakers P.J., Billiet H.A.H., de Galan L., Chromatogr J., 13, (1981)

[6]

O. Sinanoglu, B. Pullman, Molecular Association in Biology, Academic Press, New York, 1968

[7]

C. Horvath, W. Melander, I. Molnár, J. Chromatogr. 125: 129 (1976)

[8]

Horvath, W. Melander, J. Chromatogr C., 15, (1977)

[9]

Press, 9. W. R. Melander, C. Horvath, High Performance Liquid Chromatography, Advances and Perspectives Vol. 2, Academic New York, 1980, pp 113–319

[10]

F. Dowell, D. E. Martire, J. Chem. Phys., 69: 2332, (1978)

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