IMMOBILIZATION OF BETA-D-GALACTOSIDASE FROM ESCHERICHIA-COLI ON CELLULOSE BEADS AND ITS USE FOR THE SYNTHESIS OF DISACCHARIDE DERIVATIVES

被引：12

作者：

KERY, V ^{[1
]}

KUCAR, S ^{[1
]}

MATULOVA, M ^{[1
]}

HAPLOVA, J ^{[1
]}

机构：

[1] SLOVAK ACAD SCI, INST CHEM, CS-84238 BRATISLAVA, CZECHOSLOVAKIA

来源：

CARBOHYDRATE RESEARCH | 1991年 / 209卷

关键词：

D O I：

10.1016/0008-6215(91)80146-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-D-Galactosidase, isolated from cloned E. coli, was immobilised on cellulose beads via oxidation with sodium periodate, activation by cyanuric chloride, or diazotisation. beta-D-Galactosidase immobilised via azo bonds showed the highest relative activity and thermostability, and was used for synthesis of disaccharide methyl glycosides.

引用

页码：83 / 87

页数：5

共 13 条

[1] THE SYNTHESIS OF OLIGOSACCHARIDES BY THE REVERSED HYDROLYSIS REACTION OF BETA-GLUCOSIDASE AT HIGH SUBSTRATE CONCENTRATION AND AT HIGH-TEMPERATURE
AJISAKA, K
NISHIDA, H
FUJIMOTO, H
[J]. BIOTECHNOLOGY LETTERS, 1987, 9 (04) : 243 - 248
[2] AJISAKA K, 1988, CARBOHYD RES, V167, P95
[3] C-13 NUCLEAR MAGNETIC-RESONANCE DATA FOR OLIGOSACCHARIDES
BOCK, K
PEDERSEN, C
PEDERSEN, H
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1984, 42 : 193 - 225
[4] GINSBURG V, 1984, BIOL CARBOHYDRATES, V2
[5] GLYCOSPHINGOLIPIDS IN CELLULAR INTERACTION, DIFFERENTIATION, AND ONCOGENESIS
HAKOMORI, SI
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1981, 50 : 733 - 764
[6] SYNTHESIS OF GAL-BETA-1-3GLCNAC AND GAL-BETA-1-3GLCNAC-BETA-SET BY AN ENZYMATIC METHOD COMPRISING THE SEQUENTIAL USE OF BETA-GALACTOSIDASES FROM BOVINE TESTES AND ESCHERICHIA-COLI
HEDBYS, L
JOHANSSON, E
MOSBACH, K
LARSSON, PO
GUNNARSSON, A
SVENSSON, S
LONN, H
[J]. GLYCOCONJUGATE JOURNAL, 1989, 6 (02) : 161 - 168
[7] SYNTHESIS OF 2-ACETAMIDO-2-DEOXY-3-O-BETA-D-GALACTOPYRANO-SYL-D-GALACTOSE BY THE SEQUENTIAL USE OF BETA-D-GALACTO-SIDASES FROM BOVINE TESTES AND ESCHERICHIA-COLI
HEDBYS, L
JOHANSSON, E
MOSBACH, K
LARSSON, PO
GUNNARSSON, A
SVENSSON, S
[J]. CARBOHYDRATE RESEARCH, 1989, 186 (02) : 217 - 223
[8] SYNTHESIS OF MANNOSE OLIGOSACCHARIDES VIA REVERSAL OF THE ALPHA-MANNOSIDASE REACTION
JOHANSSON, E
HEDBYS, L
LARSSON, PO
MOSBACH, K
GUNNARSSON, A
SVENSSON, S
[J]. BIOTECHNOLOGY LETTERS, 1986, 8 (06) : 421 - 424
[9] KERY V, 1990, J CHEM TECHNOL BIOT, V48, P201
[10] PURIFICATION AND KINETICS OF BETA-D-GALACTOSIDASE FROM ESCHERICHIA-COLI, STRAIN-K-12
KUBY, SA
LARDY, HA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1953, 75 (04) : 890 - 896

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