LOCATION AND QUANTIFICATION OF METAL-IONS IN ENZYMES COMBINING POLYACRYLAMIDE-GEL ELECTROPHORESIS AND PARTICLE-INDUCED X-RAY-EMISSION

被引：22

作者：

SZOKEFALVINAGY, Z ^{[1
]}

BAGYINKA, C ^{[1
]}

DEMETER, I ^{[1
]}

KOVACS, KL ^{[1
]}

QUYNH, LH ^{[1
]}

机构：

[1] HUNGARIAN ACAD SCI,CTR BIOL RES,INST BIOPHYS,H-6701 SZEGED,HUNGARY

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 1990年 / 26-7卷

关键词：

bacterial hydrogenase; metalloenzyme; metalloprotein; particle-induced X-ray emission; PIXE; polyacrylamide gel electrophoresis (PAGE); trace element analysis;

D O I：

10.1007/BF02992662

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented to identify and determine the relative amounts of protein-bound metal ions in situ. Proteins or their subunits are directly scanned by a collimated proton beam of 3 MeV energy, and the characteristic X-rays produced are detected. The determination of Fe content of an iron-sulfur protein (HiPiP), as well as the Fe and Ni analysis of the hydrogenese from Thiocapsa roseopersina, have shown the feasibility of this technique. © 1990 Humana Press Inc.

引用

页码：93 / 101

页数：9

共 12 条

[1]

ADAMS MWW, 1981, BIOCHIM BIOPHYS ACTA, V594, P105

[2] UNCONSIDERED FACTORS AFFECTING HYDROGENASE ACTIVITY MEASUREMENT [J].

BAGYINKA, C ;

ZORIN, NA ;

KOVACS, KL .

ANALYTICAL BIOCHEMISTRY, 1984, 142 (01) :7-15

[3] LOCALIZATION OF HYDROGENASE IN THIOCAPSA-ROSEOPERSICINA PHOTOSYNTHETIC MEMBRANE [J].

BAGYINKA, C ;

KOVACS, KL ;

RAK, E .

BIOCHEMICAL JOURNAL, 1982, 202 (01) :255-258

[4]

CISZEWSKA G, 1984, P INT S BIOL SOLAR E, P330

[5]

GOGOTOV I N, 1976, Biokhimiya, V41, P836

[6] NITROGEN DETERMINATION WITH EXTERNAL BEAM PROTON-INDUCED GAMMA-RA EMISSION ANALYSIS [J].

HANNINEN, R ;

RAISANEN, J .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1984, 5 (01) :34-38

[7]

JORGENSON JW, 1986, ANAL CHEM, V58, P743

[8] ELEMENTAL ANALYSIS OF SAMPLES OF BIOLOGICAL ORIGIN RELATIVE TO THEIR PROTEIN-CONTENT BY MEANS OF CHARGED-PARTICLE BOMBARDMENT [J].

KESZTHELYI, L ;

VARGA, L ;

DEMETER, I ;

HOLLOSNAGY, K ;

SZOKEFALVINAGY, Z .

ANALYTICAL BIOCHEMISTRY, 1984, 139 (02) :418-426

[9] PURIFICATION OF HYDROGENASE BY FAST PROTEIN LIQUID-CHROMATOGRAPHY AND BY CONVENTIONAL SEPARATION TECHNIQUES - A COMPARATIVE-STUDY [J].

KOVACS, KL ;

TIGYI, G ;

ALFONZ, H .

PREPARATIVE BIOCHEMISTRY, 1985, 15 (05) :321-334

[10] IMMUNOLOGICAL RELATIONSHIP AMONG HYDROGENASES [J].

KOVACS, KL ;

SEEFELDT, LC ;

TIGYI, G ;

DOYLE, CM ;

MORTENSON, LE ;

ARP, DJ .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :430-435

← 1 2 →