A HETEROGENEOUS DOUBLE ANTIBODY ENZYME-LINKED IMMUNOASSAY TO MEASURE BETA-GALACTOSIDASE FUSION PROTEIN

被引：3

作者：

HAYASHIBE, K

SASSANO, D

ZIAI, MR

机构：

[1] AMER CYANAMID CO,DIV MED RES,CENT NERVOUS SYST RES DEPT,PEARL RIVER,NY 10965

[2] KOBE UNIV,DEPT DERMATOL,KOBE 657,JAPAN

来源：

JOURNAL OF IMMUNOASSAY | 1990年 / 11卷 / 01期

关键词：

D O I：

10.1080/01971529008053260

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive Enzyme&linked immunoassay (ELISA) to quantitate recombinant fusion proteins encoded by cloned cDNA in the bacteriophage λgtl is described. Since the fusion protein is expressed in an equimolar ratio toß-galactosidase, the assay derives the concentration of the recombinant protein in total bacterial lysates or pure preparations from the measurement of p-galactosidase with an Enzyme&linked immunoassay. This assay is a useful technique to measure the recombinant proteins for subsequent immunological and biochemical characterization. (KEYWORDS: λgtl 1, fusion protein, ELISA). © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：89 / 95

页数：7