STRUCTURE AND DYNAMICS OF LIPID MONOLAYERS - IMPLICATIONS FOR ENZYME-CATALYZED LIPOLYSIS

被引：34

作者：

PETERS, GH

TOXVAERD, S

LARSEN, NB

BJORNHOLM, T

SCHAUMBURG, K

KJAER, K

机构：

[1] NOVO NORDISK AS, DK-2880 BAGSVAERD, DENMARK

[2] UNIV COPENHAGEN, CTR INTERDISCIPLINARY STUDIES MOLEC INTERACT, DEPT CHEM, DK-2100 COPENHAGEN, DENMARK

[3] RISO NATL LAB, DEPT PHYS, DK-4000 ROSKILDE, DENMARK

来源：

NATURE STRUCTURAL BIOLOGY | 1995年 / 2卷 / 05期

关键词：

D O I：

10.1038/nsb0595-395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have investigated the role of the substrate on the interfacial activation of lipases by an interdisciplinary study of the structure and dynamics of 1,2-sn dipalmitoylglycerol monolayers at distinct surface pressures. The diglyceride Langmuir film undergoes two phase transitions occurring at 38.3 and 39.8 Angstrom(2) per molecule. The first transition is unique for diglyceride molecules and is driven by a reorganization of the headgroups causing a change in the hydrophobicity of the oil-water interface. X-ray diffraction studies of different mesophases shows that in the two highest pressure phases, the alkyl chains pack in an hexagonal structure relaxing to a distorted-hexagonal lattice in the lowest pressure phase with the alkyl chains tilted by similar to 14 degrees in a direction close to a nearest neighbour direction.

引用

页码：395 / 401

页数：7

共 36 条

[1]

Derewenada U., Et al., Conformational lability of lipases observed in the absence of an oil-water Interface: Crystallograhlc studies of enzymes from fungi humicola lanuginosa and rhizopus delemar, J. Lipid Res., 35, pp. 524-534, (1994)

[2]

Brzozowski A.M., A model for Interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex, Nature, 351, pp. 491-494, (1991)

[3]

Brockman H.L.U., 145, (1984)

[4]

Muderhwa J.M., Brockman H.L., Lateral lipid distribution is a major regulator of lipase activity, J. Biol. Chem, 267, pp. 24184-24192, (1992)

[5]

Van Tilbeurgh H., Egloff M.P., Martinez C., Rugani N., Verger R., Camblllau C., Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography, Nature, 362, pp. 814-820, (1993)

[6]

Verger R., Regulation by the "interfacial quality" of some biological activities, Colloids and Surfaces, 10, pp. 163-180, (1984)

[7]

Thuren T., A model for the molecular mechanism of interfacial activation of phospholipase A2 supporting the substrate theory, FEBS Lett, 229, pp. 95-99, (1988)

[8]

Pibronl G., Gargourl Y., Sarda L., Verger R., Interactions of lipases with lipid monolayers. Facts and questions, Adv. Colloid Interface. Sci, 32, pp. 341-378, (1990)

[9]

Knobler C.M., Desai R.C., Phase transitions In monolayers, A. Rev. Phys. Chem, 43, pp. 207-236, (1992)

[10]

Knobler C.M., Recent developments in the study of monolayers at the air-water Interface, Advances in Chemical Physics, pp. 397-449, (1990)

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