UDP-GLUCURONYLTRANSFERASE ACTIVITY TOWARD HARMOL IN HUMAN LIVER AND HUMAN FETAL LIVER-CELLS IN CULTURE

被引:12
作者
TAN, TMC
SIT, KH
WONG, KP
机构
[1] NATL UNIV SINGAPORE, DEPT BIOCHEM, 10 KENT RIDGE CRESCENT, SINGAPORE 0511, SINGAPORE
[2] NATL UNIV SINGAPORE, DEPT ANAT, SINGAPORE 0511, SINGAPORE
关键词
D O I
10.1016/0003-2697(90)90252-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper presents a fast HPLC assay for measuring UDP-glucuronyltransferase (UDPGT) activity in extracts of adult human liver and human fetal liver cells in culture. Harmol glucoronide formed was quantitated directly without prior hydrolysis after a simple step of selective extraction of harmol. The method is applicable to crude liver homogenates as well as to partially fractionated preparations. It is several fold more sensitive than the conventional detection of harmol glucuronide by TLC, making it possible to distinguish the low and high affinity forms of UDPGT of adult human liver and to detect the low affinity form in fetal cells. The possible participation of both forms of GT in adults under different conditions and the apparent lack of the high affinity form in the fetal liver is discussed. © 1990.
引用
收藏
页码:44 / 50
页数:7
相关论文
共 22 条
[1]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF HARMOL AND ITS CONJUGATED METABOLITES AFTER ENZYME HYDROLYSIS IN BIOLOGICAL-FLUIDS [J].
CHING, MS ;
MIHALY, GW ;
ANGUS, PW ;
ANDERSON, JD ;
SMALLWOOD, RA .
JOURNAL OF CHROMATOGRAPHY, 1986, 380 (01) :190-195
[2]   COMPARATIVE-STUDY OF CLOFIBRIC ACID AND BILIRUBIN GLUCURONIDATION IN HUMAN-LIVER MICROSOMES [J].
DRAGACCI, S ;
HAMARHANSEN, C ;
FOURNELGIGLEUX, S ;
LAFAURIE, C ;
MAGDALOU, J ;
SIEST, G .
BIOCHEMICAL PHARMACOLOGY, 1987, 36 (22) :3923-3927
[3]  
GREENBLATT DJ, 1976, CLIN PHARMACOL THER, V20, P329
[4]  
IRSHAID YM, 1987, MOL PHARMACOL, V31, P27
[5]   EFFECT OF DOSE ON THE GLUCURONIDATION AND SULFATION KINETICS OF DIFLUNISAL IN MAN - SINGLE DOSE STUDIES [J].
LOEWEN, GR ;
HERMAN, RJ ;
ROSS, SG ;
VERBEECK, RK .
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1988, 26 (01) :31-39
[6]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[7]   KINETIC AND INHIBITOR STUDIES OF 4-METHYLUMBELLIFERONE AND 1-NAPHTHOL GLUCURONIDATION IN HUMAN-LIVER MICROSOMES [J].
MINERS, JO ;
LILLYWHITE, KJ ;
MATTHEWS, AP ;
JONES, ME ;
BIRKETT, DJ .
BIOCHEMICAL PHARMACOLOGY, 1988, 37 (04) :665-671
[8]   INVITRO EVIDENCE FOR THE INVOLVEMENT OF AT LEAST 2 FORMS OF HUMAN-LIVER UDP-GLUCURONOSYLTRANSFERASE IN MORPHINE 3-GLUCURONIDATION [J].
MINERS, JO ;
LILLYWHITE, KJ ;
BIRKETT, DJ .
BIOCHEMICAL PHARMACOLOGY, 1988, 37 (14) :2839-2845
[9]   EXTRAHEPATIC SULFATION AND GLUCURONIDATION IN THE RAT INVIVO - DETERMINATION OF THE HEPATIC EXTRACTION RATIO OF HARMOL AND THE EXTRAHEPATIC CONTRIBUTION TO HARMOL CONJUGATION [J].
MULDER, GJ ;
WEITERING, JG ;
SCHOLTENS, E ;
DAWSON, JR ;
PANG, KS .
BIOCHEMICAL PHARMACOLOGY, 1984, 33 (19) :3081-3087
[10]   UDP GLUCURONYLTRANSFERASE AND PHENOLSULFOTRANSFERASE INVIVO AND INVITRO - CONJUGATION OF HARMOL AND HARMALOL [J].
MULDER, GJ ;
HAGEDOORN, AH .
BIOCHEMICAL PHARMACOLOGY, 1974, 23 (15) :2101-2109