MAPPING OF INTRACELLULAR HALOGENOUS MOLECULES BY LOW AND HIGH-RESOLUTION SIMS MICROSCOPY

被引:9
作者
BERRY, JP [1 ]
GALLE, P [1 ]
CHASSOUX, D [1 ]
ESCAIG, F [1 ]
LINAREZCRUZ, LG [1 ]
LESPINATS, G [1 ]
机构
[1] INST RES SCI CANC,F-94805 VILLEJUIF,FRANCE
关键词
SIMS MICROSCOPY; HALOGENOUS MOLECULES; TUMOR CELLS; INTRACELLULAR LOCALIZATION;
D O I
10.1016/0248-4900(92)90013-Q
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The subcellular distribution of halogenous molecules has been studied by SIMS microscopy in cultured cells of a human breast carcinoma (MCF-7 cell line). Two instruments of microanalysis were used. A low lateral resolution ion microscope (SMI 300 CAMECA) and a prototype scanning ion microscope equipped with a cesium gun that gives high lateral resolution images. This apparatus has been developed by G Slodzian, in Onera Laboratories (Office National d'Etudes et de Recherches Aerospatiales). Molecules studied by low lateral resolution ion microscope were halogenous steroids: fluorometholone, triamcinolone, bromocriptine and bromoandrosterone. Analytical images show that the first two compounds are mainly localized in the nuclear structure of MCF-7 cells whereas the last two molecules are localized in cytoplasm of these cells. Images were obtained with a resolution of 1-mu-m. With the scanning ion microscope, it is now possible to obtain images at the ultrastructural level. Four analytical images can be simultaneously obtained by a single scan of the imaged area, corresponding to a depth of erosion of the section of ten nm. The intranuclear distributions of three pyrimidine analogs, 5-bromo-2'-deoxyuridine, 5-iodo-2'-deoxyuridine and 5-fluorouracil have been studied in phase S and M of MCF-7 cells and these images have been compared to the distribution of sulfur, nitrogen and phosphorus. All these images have been obtained with a lateral resolution better that 100 nm.
引用
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页码:93 / 98
页数:6
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