INVERTED MICROCARRIERS FOR CELL-CULTURES MADE BY POLYMERIZATION OF SHELLS AROUND AGAROSE MICROSPHERES IN A NONCYTOTOXIC PROCEDURE

被引：5

作者：

CADIC, C ^{[1
]}

BAQUEY, C ^{[1
]}

DUPUY, B ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,INSERM,U 306,F-33076 BORDEAUX,FRANCE

来源：

POLYMER JOURNAL | 1991年 / 23卷 / 08期

关键词：

MICROENCAPSULATION; MICROCARRIERS; CELL CULTURE; SEEDED POLYMERIZATION;

D O I：

10.1295/polymj.23.933

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A new process for microencapsulating cells is described. Cells in solution in agarose were extruded from a syringe into a paraffin oil containing medium. Solid microspheres were produced when the agarose gelled. A rigid interface around each bead was produced by polymerization of a latex seeded solution of a mixture of 5% acrylamide and 0.25% bisacrylamide. Cell viability was demonstrated by radioimmunoassay of prolactin liberated from encapsulated pituitary cells.

引用

页码：933 / 937

页数：5

共 10 条

[1] PREVENTION OF COMPLEMENT-MEDIATED CYTOTOXICITY AGAINST RAT ISLETS BY ENCAPSULATION IN A CELLULOSE SULFATE MEMBRANE [J].