AN ANTICOAGULANT DERMATAN SULFATE PROTEOGLYCAN CIRCULATES IN THE PREGNANT WOMAN AND HER FETUS

被引:66
作者
ANDREW, M
MITCHELL, L
BERRY, L
PAES, B
DELORME, M
OFOSU, F
BURROWS, R
KHAMBALIA, B
机构
[1] MCMASTER UNIV,DEPT PATHOL,HAMILTON L8N 3Z5,ONTARIO,CANADA
[2] MCMASTER UNIV,DEPT OBSTET & GYNECOL,HAMILTON L8N 3Z5,ONTARIO,CANADA
关键词
GLYCOSAMINOGLYCAN; HEPARIN COFACTOR-II; PERINATAL; THROMBIN;
D O I
10.1172/JCI115579
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Investigation of the in vitro ability of plasma from pregnant women to inhibit exogenous thrombin (25 nM) demonstrated that heparin cofactor II inhibited more thrombin (3.0 +/- 0.7 nM, mean +/- SD) than plasma from women 3-5 d postpartum (1.9 +/- 0.5 nM) or plasma from nonpregnant adults (1.5 +/- 0.4 nM). Levels of heparin cofactor II were only slightly increased over normal in both pregnant and postpartum women and did not account for the observed increase in thrombin bound to heparin cofactor II. Assay of pregnancy plasma for dermatan sulfate anticoagulant activity demonstrated the presence of activity equivalent to 0.23 +/- 0.02-mu-g/ml of porcine mucosal dermatan sulfate. This activity could not be demonstrated in normal adult plasma or plasma from women on the contraceptive pill. The mass of dermatan sulfate in pregnancy and umbilical cord plasmas was increased over adult control plasma by 0.20-mu-g/ml (53%) and 0.29-mu-g/ml (76%), respectively. The glycosaminoglycan-containing fraction of plasma was isolated and an assay for anticoagulant dermatan sulfate confirmed its presence in both pregnancy and cord plasmas but minimal activity in adult plasma. Gel chromatography of isolated fractions from both pregnancy and cord plasmas revealed a polydisperse, active species with apparent M(r) 150,000 D. Reductive elimination decreased the apparent M(r) of the active species on gel chromatography to 31,000 D for cord and 21,000 D for pregnancy products. This confirmed the presence of an anticoagulant active dermatan sulfate proteoglycan circulating in the plasmas of pregnant women at term and fetuses at delivery.
引用
收藏
页码:321 / 326
页数:6
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