PURIFICATION OF N-METHYLPUTRESCINE OXIDASE FROM NICOTIANA-RUSTICA

被引：8

作者：

HASLAM, SC

YOUNG, TW

机构：

[1] School of Biochemistry, The University of Birmingham, Edgbaston, Birmingham

来源：

PHYTOCHEMISTRY | 1992年 / 31卷 / 12期

关键词：

NICOTIANA-RUSTICA; SOLANACEAE; TOBACCO; N-METHYLPUTRESCINE; OXIDASE; ENZYME PURIFICATION;

D O I：

10.1016/0031-9422(92)80417-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The yield of N-methylputrescine oxidase extracted from Nicotiana rustica roots grown in organ culture was improved by supplementing White's medium with 1.15 mM (NH4)2SO4. The enzyme was purified from these roots by salt fractionation, anion exchange, affinity and hydrophobic interaction chromatography. The purified protein had M(r) 54000 by SDS-PAGE.

引用

页码：4075 / 4079

页数：5

共 9 条

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