CHARACTERIZATION OF PSEUDORABIES VIRUS GLYCOPROTEIN GII EXPRESSED BY RECOMBINANT BACULOVIRUS

被引：26

作者：

XUAN, X

NAKAMURA, T

IHARA, T

SATO, I

TUCHIYA, K

NOSETTO, E

ISHIHAMA, A

UEDA, S

机构：

[1] NIPPON INST BIOL SCI,OME,TOKYO 198,JAPAN

[2] UNIV TOKYO,FAC AGR,DEPT VET MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN

[3] NATL UNIV LA PLATA,FAC VET SCI,INST VIROL,CONICET,RA-1900 LA PLATA,ARGENTINA

[4] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

来源：

VIRUS RESEARCH | 1995年 / 36卷 / 2-3期

关键词：

PSEUDORABIES VIRUS; GLYCOPROTEIN GII; N-TERMINAL SEQUENCE; EXPRESSION; BACULOVIRUS;

D O I：

10.1016/0168-1702(94)00112-P

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene encoding the complete glycoprotein gII (homologue of gB of herpes simplex virus) of pseudorabies virus (PrV) was inserted into a baculovirus transfer vector, and a recombinant virus expressing gII was isolated. Three gII-related recombinant baculovirus-expressed peptides of 100, 60, and 45 to 50 kDa were detected with a polyclonal antibody against gII; these correspond to the authentic subunits gIIa and its cleavage products gIIb and gIIc, respectively. These proteins were subjected to N-terminal sequencing, and the results showed that the protease cleavage sites were identical to those of authentic gII. The expressed gII was shown to be transported to the surface of infected cells as judged by an indirect immunofluorescence test. Antibodies raised in mice immunized with the recombinant gII neutralized the infection of PrV in vitro. Mice inoculated with the recombinant gII were completely protected from lethal challenge with PrV.

引用

页码：151 / 161

页数：11

共 22 条

[1]

Ghiasi, Kaiwar, Nesburn, Wechsler, Expression of herpes simplex virus type 1 glycoprotein B in insect cells, Virus Res., 22, pp. 25-39, (1991)

[2]

Ghiasi, Kaiwar, Nesburn, Slanina, Wechsler, Expression of seven herpes simplex virus type 1 glycoproteins (gB, gC, gD, gE, gG, gH, and gI): comparative protection against lethal challenge in mice, J. Virol., 68, pp. 2118-2126, (1994)

[3]

Hampl, Ben-Porat, Ehrlicher, Habermehl, Kaplan, Characterization of the envelope proteins of pseudorabies virus, J. Virol., 52, pp. 583-590, (1984)

[4]

Horimoto, Limcumpao, Tohya, Takahashi, Mikami, Feline herpesvirus type 1 glycoproteins eliciting virus neutralizing and hemagglutination-inhibiting antibodies, Arch. Virol., 111, pp. 127-132, (1990)

[5]

King, Possee, The Baculovirus Expression System, (1992)

[6]

Kopp, Blewett, Misra, Mettenleiter, Proteolytic cleavage of bovine herpesvirus 1 (BHV-1) glycoprotein gB is not necessary for its function in BHV-1 or pseudorabies virus, J. Virol., 68, pp. 1667-1674, (1994)

[7]

Lukacs, Thiel, Mettenleiter, Rziha, Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein complex, J. Virol., 53, pp. 166-173, (1985)

[8]

Maniatis, Fritsch, Sambrook, Molecular Cloning: A Laboratory Manual, (1982)

[9]

Matsuura, Possee, Bishop, Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins, J. Gen. Virol., 68, pp. 1233-1250, (1987)

[10]

Matsuura, Miyamoto, Sato, Morita, Yasui, Characterization of Japanese encephalitis virus envelope proteine xpressed by recombinant baculoviruses, Virology, 173, pp. 390-399, (1989)

← 1 2 3 →