IDENTIFICATION OF SOME BACTERIAL-CELL COMPONENTS BY FT-IR SPECTROSCOPY

被引：138

作者：

HELM, D

NAUMANN, D

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 126卷 / 01期

关键词：

INFRARED SPECTROSCOPY; SPECTROSCOPIC MARKER BAND; POLYPEPTIDE CAPSULE; ENDOSPORE; BACILLUS;

D O I：

10.1111/j.1574-6968.1995.tb07393.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Some particular cell components can be detected and identified by Fourier Transform infrared spectroscopy (FT-IR) of intact bacteria. Typical marker bands were used to identify these bacterial cell components. Polypeptide capsules were detected in several Bacillus species by a band typical for alpha-helical structures and by strong carboxylate stretching vibrations. Formation of endospores in clostridia and bacilli was discovered using marker bands for dipicolinic acid. Spectra of some Bacillus strains showed expression of poly-beta-hydroxybutyric acid granules, capsules and endospores simultaneously.

引用

收藏

页码：75 / 79

页数：5

相关论文

共 25 条

[1] BIOFILMS, THE CUSTOMIZED MICRONICHE [J].

COSTERTON, JW ;

LEWANDOWSKI, Z ;

DEBEER, D ;

CALDWELL, D ;

KORBER, D ;

JAMES, G .

JOURNAL OF BACTERIOLOGY, 1994, 176 (08) :2137-2142

[2]

CURK MC, 1994, FEMS MICROBIOL LETT, V123, P241, DOI 10.1016/0378-1097(94)90204-6

[3]

GARY ND, 1959, J BACTERIOL, V76, P359

[4] A NOVEL, HIDDEN PENICILLIN-INDUCED DEATH OF STAPHYLOCOCCI AT HIGH DRUG CONCENTRATION, OCCURRING EARLIER THAN MUROSOME-MEDIATED KILLING PROCESSES [J].

GIESBRECHT, P ;

KERSTEN, T ;

MAIDHOF, H ;

KRUGER, D ;

BLUMEL, P ;

GROB, H ;

WECKE, J .

ARCHIVES OF MICROBIOLOGY, 1994, 161 (05) :370-383

[5] RAPID DIFFERENTIATION OF ARCHAEBACTERIA FROM EUBACTERIA BY DIFFUSE REFLECTANCE FOURIER-TRANSFORM IR SPECTROSCOPIC ANALYSIS OF LIPID PREPARATIONS [J].

HEDRICK, DB ;

NIVENS, DE ;

STAFFORD, C ;

WHITE, DC .

JOURNAL OF MICROBIOLOGICAL METHODS, 1991, 13 (01) :67-73

[6] CLASSIFICATION AND IDENTIFICATION OF BACTERIA BY FOURIER-TRANSFORM INFRARED-SPECTROSCOPY [J].

HELM, D ;

LABISCHINSKI, H ;

SCHALLEHN, G ;

NAUMANN, D .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :69-79

[7] ELABORATION OF A PROCEDURE FOR IDENTIFICATION OF BACTERIA USING FOURIER-TRANSFORM IR SPECTRAL LIBRARIES - A STEPWISE CORRELATION APPROACH [J].

HELM, D ;

LABISCHINSKI, H ;

NAUMANN, D .

JOURNAL OF MICROBIOLOGICAL METHODS, 1991, 14 (02) :127-142

[8] SIMULTANEOUS INFECTIONS WITH DIFFERENT SEROGROUPS OF LEGIONELLA-PNEUMOPHILA INVESTIGATED BY ROUTINE METHODS AND FOURIER-TRANSFORM INFRARED-SPECTROSCOPY [J].

HORBACH, I ;

NAUMANN, D ;

FEHRENBACH, FJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (06) :1106-1110

[9] THE SLIME OF COAGULASE-NEGATIVE STAPHYLOCOCCI - BIOCHEMISTRY AND RELATION TO ADHERENCE [J].

HUSSAIN, M ;

WILCOX, MH ;

WHITE, PJ .

FEMS MICROBIOLOGY LETTERS, 1993, 104 (3-4) :191-208

[10] BACTERIAL IDENTIFICATION BY INFRARED SPECTROPHOTOMETRY .2. EFFECT OF INSTRUMENTAL AND ENVIRONMENTAL VARIABLES [J].

KENNER, BA ;

RIDDLE, JW ;

ROCKWOOD, SW ;

BORDNER, RH .

JOURNAL OF BACTERIOLOGY, 1958, 75 (01) :16-20