RAPID GENOTYPING OF LOW-DENSITY-LIPOPROTEIN RECEPTOR KNOCKOUT MICE USING A POLYMERASE CHAIN-REACTION TECHNIQUE

被引：24

作者：

GAW, A

MANCINI, FP

ISHIBASHI, S

机构：

[1] UNIV TEXAS,SW MED CTR,DEPT MOLEC GENET,DALLAS,TX 75235

[2] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,TOKYO 113,JAPAN

来源：

LABORATORY ANIMALS | 1995年 / 29卷 / 04期

关键词：

MICE; LDL RECEPTOR; PCR; GENOTYPING;

D O I：

10.1258/002367795780739944

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A new method for the rapid genotyping of low density lipoprotein receptor knockout mice is described. This method using a polymerase chain reaction (PCR) technique may be performed on small tissue biopsies, and represents a significant advantage in time over conventional genotyping by Southern blot analyses.

引用

页码：447 / 449

页数：3

共 5 条

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[2] Goldstein J.L., 1989, METABOLIC BASIS INHE, P1215
[3] GORDON JW, 1993, GUIDE TECHNIQUES MOU
[4] HYPERCHOLESTEROLEMIA IN LOW-DENSITY-LIPOPROTEIN RECEPTOR KNOCKOUT MICE AND ITS REVERSAL BY ADENOVIRUS-MEDIATED GENE DELIVERY
ISHIBASHI, S
BROWN, MS
GOLDSTEIN, JL
GERARD, RD
HAMMER, RE
HERZ, J
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (02) : 883 - 893
[5] MASSIVE XANTHOMATOSIS AND ATHEROSCLEROSIS IN CHOLESTEROL-FED LOW-DENSITY-LIPOPROTEIN RECEPTOR-NEGATIVE MICE
ISHIBASHI, S
GOLDSTEIN, JL
BROWN, MS
HERZ, J
BURNS, DK
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (05) : 1885 - 1893

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