ANALYSIS OF OPA-DERIVATIZED AMINO-SUGARS IN TOBACCO BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION

被引：8

作者：

DOMINGUEZ, LM

DUNN, RS

机构：

[1] R.J. Reynolds Tobacco Company, Bowman Gray Technical Center, Winston-Salem, NC, 27102, United States

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1987年 / 25卷 / 10期

关键词：

CHROMATOGRAPHIC ANALYSIS - Liquid - TOBACCO - Chromatographic Analysis;

D O I：

10.1093/chromsci/25.10.468

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method is developed to quantitatively determine glucosamine, galactosamine, and mannosamine in dried-and-ground burley and flue-cured tobaccos. Extraction is shown to be quantitative in the range of 0. 01 to 2. 0% (w/w). The OPA derivatives of the aminosugars are then detected with a fluorimetric detector, and their simultaneous analysis is performed with an external standard. This method is shown to be selective, accurate, and precise.

引用

页码：468 / 471

页数：4

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