A LONG-RANGE RESTRICTION MAP BETWEEN THE ALPHA-GLOBIN COMPLEX AND A MARKER CLOSELY LINKED TO THE POLYCYSTIC KIDNEY DISEASE-1 (PKD1) LOCUS

被引：49

作者：

HARRIS, PC ^{[1
]}

BARTON, NJ ^{[1
]}

HIGGS, DR ^{[1
]}

REEDERS, ST ^{[1
]}

WILKIE, AOM ^{[1
]}

机构：

[1] YALE UNIV,SCH MED,DEPT NEPHROL,NEW HAVEN,CT 06510

来源：

GENOMICS | 1990年 / 7卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0888-7543(90)90541-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two polymorphic loci and two additional probes that map close to CMM65, which is tightly linked to the polycystic kidney disease 1 (PKD1) locus in chromosome band 16p13.3, are described. These new probes were isolated from a library that was enriched by preparative pulsed-field gel electrophoresis (PFGE) for sequences from a 320-kb NotI fragment that includes CMM65. Through the use of a panel of somatic cell hybrids and PFGE, the new polymorphic loci, PNL56S and NKISP1, were localized within 60 kb and approximately 250 kb distal to CMM65, respectively. A long-range restriction map linking these new probes and the distal markers EKMDA2, CMM103, and α-globin was constructed. These latter probes have been localized to regions approximately 900 kb, 1.2 Mb, and 1.9 Mb distal to CMM65, respectively. The entire region was found to be unusually rich in CpG dinucleotides. The new polymorphic probes and the long-range map will aid both the search for the PKD1 locus and the detailed characterization of this distal region of 16p. © 1990.

引用

页码：195 / 206

页数：12

共 45 条

[1] CLONES FROM AN 840-KB FRAGMENT CONTAINING THE 5' REGION OF THE DMD LOCUS ENRICHED BY PULSED FIELD GEL-ELECTROPHORESIS [J].