CLONING AND SEQUENCING OF THE GENE ENCODING CYTOCHROME-C-551 FROM PSEUDOMONAS-AERUGINOSA

被引：40

作者：

ARAI, H ^{[1
]}

SANBONGI, Y ^{[1
]}

IGARASHI, Y ^{[1
]}

KODAMA, T ^{[1
]}

机构：

[1] UNIV TOKYO,DEPT AGR CHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1990年 / 261卷 / 01期

关键词：

(Pseudomonas aeruginosa); Cytochrome c-551; DNA sequence; Nitrite reductase; Signal peptide;

D O I：

10.1016/0014-5793(90)80669-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cytochrome c-551 gene from Pseudomonas aeruginosa was cloned by using two oligonucleotide probes, which had been synthesized based on the known primary structure of the protein. The restriction map of the cloned DNA and sequence analysis showed that the cytochrome c-551 gene is located 50 bp downstream of the nitrite reductase gene, which has recently been cloned and sequenced. DNA sequence analysis also indicated that cytochrome c-551 is synthesized in vivo as a precursor having an amino-terminal signal sequence consisting of 22 amino acid residues. © 1990.

引用

页码：196 / 198

页数：3

共 19 条

[1] AMINO ACID SEQUENCE OF PSEUDOMONAS CYTOCHROME C-551 [J].