PERTUSSIS TOXIN TREATMENT MODIFIES OPIATE ACTION IN THE RAT-BRAIN STRIATUM

被引：48

作者：

ABOOD, ME ^{[1
]}

LAW, PY ^{[1
]}

LOH, HH ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, LANGLEY PORTER NEUROPSYCHIAT INST, SAN FRANCISCO, CA 94143 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 127卷 / 02期

关键词：

D O I：

10.1016/S0006-291X(85)80185-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Evidence is presented that a guanine nucleotide regulatory protein, Gi, mediates opiate action in the rat brain striatum. Opiates inhibit basal adenylate cyclase activity in rat brain striatum. This effect on adenylate cyclase is dose-dependently attenuated by pretreatment of membranes with pertussis toxin, which ADP-ribosylates a protein with a molecular mass of 41,000 daltons. This protein comigrates with the GTP-binding subunit of Gi, which mediates inhibition of adenylate cyclase. Several brain regions were compared for the extent of radiolabeling and effects on adenylate cyclase activity. Although Gi was found in each region examined, opiate inhibition of adenylate cyclase is clearly seen only in the striatum.

引用

页码：477 / 483

页数：7

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