EFFECT OF CAMP AND CGMP ON ENDOTHELIN-STIMULATED TYROSINE PHOSPHORYLATION IN RABBIT PLATELETS

被引：9

作者：

CATALAN, RE ^{[1
]}

MARTINEZ, AM ^{[1
]}

GARGIULO, L ^{[1
]}

LIRAS, A ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID, FAC QUIM, DEPT BIOQUIM & BIOL MOLEC 1, E-28040 MADRID, SPAIN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 215卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2456

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activation of platelets by different agents results in the increased tyrosine phosphorylation of several substrate proteins. Thus, the effect of endothelin-l on the stimulation of tyrosine phosphorylation in rabbit platelets can be inhibited by preincubation with forskolin, which increase the cAMP level. However, incubations of platelets with 8-Bromo-cGMP showed lower inhibitory effect. Forskolin produced a dose-dependent inhibition on three different protein substrates, with an IC50 of approximately 12.8, 4.0 and 8.0 mu M in the three molecular mass ranges of 50, 60 and 100-200 kDa, respectively. These results show that the endothelin-stimulated tyrosine phosphorylation in rabbit platelets can be regulated by a novel pathway of platelet signal transduction in which the cAMP level could be more relevantly involved than cGMP in some molecular mass ranges of tyrosine phosphorylated proteins. (C) 1995 Academic Press. Inc.

引用

页码：219 / 226

页数：8

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