A NEW DERIVATIZATION PROCEDURE FOR THE ANALYSIS OF HIPPURIC-ACID AND M-METHYL-HIPPURIC ACID BY GAS-CHROMATOGRAPHY

被引：18

作者：

DECARVALHO, D

LANCHOTE, VL

BONATO, PS

QUEIROZ, RHC

SANTOS, AC

DREOSSI, SAC

机构：

[1] Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo

来源：

INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH | 1991年 / 63卷 / 01期

关键词：

TOLUENE; XYLENE; OCCUPATIONAL EXPOSURE; HIPPURIC ACID; M-METHYL-HIPPURIC ACID; BIOLOGICAL MARKERS; METHANOL IN ACID MEDIUM;

D O I：

10.1007/BF00406195

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

The industrial solvents, toluene and xylene, have physicochemical properties that can be hazardous to the workers exposed. Since hippuric acid and m-methyl-hippuric acid represent the products of toluene and xylene biotransformation in urine, they are used as biological markers in studies on occupational exposure to these solvents. Several methods have been used to determine hippuric acid and m-methyl-hippuric acid - either based on gas chromatography or on high-performance liquid chromatography. In this study we propose the derivatization of hippuric acid and methyl-hippuric acid using methanol in acid medium (HCI), a low-cost reagent with a low level of toxicity. The method has been routinely used in our laboratory for 1 year and has proven to be a reliable procedure for the biological control of occupational exposure to toluene and/or xylene.

引用

页码：33 / 37

页数：5

共 18 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HIPPURIC-ACID IN HUMAN-URINE - PRELIMINARY-RESULTS FOR NORMAL URINE LEVELS [J].

ASTIER, A ;

DEUTSCH, AM .

JOURNAL OF CHROMATOGRAPHY, 1980, 182 (01) :88-93

[2]

BUCHET JP, 1973, BRIT J IND MED, V30, P125

[3]

CAMPBELL L, 1988, BRIT J IND MED, V45, P127

[4]

CAPEROS JR, 1977, BRIT J IND MED, V34, P229

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE RAPID PROFILING OF PLASMA AND URINARY ORGANIC-ACIDS [J].

CHONG, WK ;

MILLS, GA ;

WEAVIND, GP ;

WALKER, V .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 487 (01) :147-153

[6]

ELLER PM, 1984, NIOSH MANUAL ANAL ME

[7] HIPPURIC-ACID AND O-CRESOL IN THE URINE OF WORKERS EXPOSED TO TOLUENE [J].

HASEGAWA, K ;

SHIOJIMA, S ;

KOIZUMI, A ;

IKEDA, M .

INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 1983, 52 (03) :197-208

[8]

KIRA S, 1977, BRIT J IND MED, V34, P305

[9] DETERMINATION OF BENZOIC-ACID AND HIPPURIC-ACID IN HUMAN-PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

KUBOTA, K ;

HORAI, Y ;

KUSHIDA, K ;

ISHIZAKI, T .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 425 (01) :67-75

[10] SIMULTANEOUS DETERMINATION OF HIPPURIC-ACID AND URINARY ORTHO-METHYLHIPPURIC, META-METHYLHIPPURIC AND PARA-METHYLHIPPURIC ACIDS BY LIQUID-CHROMATOGRAPHY [J].

LACROIX, C ;

INGER, F ;

MENAGER, S ;

LAFONT, O .

JOURNAL OF CHROMATOGRAPHY, 1986, 382 :275-279

← 1 2 →