GAS-SUPPLY ACROSS MEMBRANES IN BIOREACTORS FOR HEPATOCYTE CULTURE

被引：48

作者：

GERLACH, J ^{[1
]}

KLOPPEL, K ^{[1
]}

STOLL, P ^{[1
]}

VIENKEN, J ^{[1
]}

MULLER, C ^{[1
]}

机构：

[1] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW CHARLOTTENBURG,INST KLIN CHEM & BIOCHEM,W-1000 BERLIN 19,GERMANY

来源：

ARTIFICIAL ORGANS | 1990年 / 14卷 / 05期

关键词：

Bioreactors; Hepatocytes; Hybrid liver support systems; Membranes; Oxygenation; Polypropylene;

D O I：

10.1111/j.1525-1594.1990.tb02976.x

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Abstract: The conditions required for hepatocyte cultures is a main topic in the development of bioreactors for hybrid liver support systems. The detoxification of ammonia and the synthesis of urea due to primary isolated hepatocytes was measured in order to compare two different models of gas supply in bioreactors: (a) indirect medium oxygenation and (b) direct membrane‐contact oxygenation of the hepatocytes using polypropylene membranes. Increasing oxygen pressure promoted cell function. At day 6 of culture, urea synthesis was 0.8 ± 0.3 raM in 21% of 02 cultures and 1.5 ± 0.1 mM in oxygenated cultures. Alkalosis due to C02 loss decreased ammonia metabolism. The direct membrane‐contact oxygenation resulted in enhanced cell metabolism in comparison to medium oxygenation: urea synthesis at day six was 1.42 ± 0.2 mM in 21% O2 cultures. Polypropylene oxygenation membranes proved to be sufficient for hepatocyte adhesion. Two functions can be integrated in one element in liver support systems using the investigated polypropylene membrane and the direct membrane‐contact oxygenation: oxygenation with physiological oxygen pressure in bioreactors due to gas supply across the membrane and adhesion of hepatocytes in bioreactors on the membrane. © 1990 International Society for Artificial Organs

引用

页码：328 / 333

页数：6

共 17 条

[1] HIGH-YIELD PREPARATION OF ISOLATED RAT LIVER PARENCHYMAL CELLS - A BIOCHEMICAL AND FINE STRUCTURAL STUDY [J].