THE EFFECTS OF INORGANIC SALTS ON THE STABILITY OF ISOCITRATE DEHYDROGENASE FROM BEEF (BOS-TAURUS) HEART AND LIVER

被引：1

作者：

BALAMIR, A ^{[1
]}

EMERK, K ^{[1
]}

机构：

[1] HACETTEPE UNIV, DEPT BIOCHEM, ANKARA, TURKEY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1982年 / 71卷 / 03期

关键词：

D O I：

10.1016/0305-0491(82)90413-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Distinct differences are established between the 2 isozymes of NADP+-dependent isocitrate dehydrogenase, purified from beef (B. taurus) heart and liver, with respect to their net electrical charges and stabilization properties in the presence of inorganic salts. Inorganic salts also inhibit the isozymes with different apparent Ki values. Both isozymes are heat labile in the absence and cold labile in the presence of salts. Different mechanisms of heat stabilization probably exist due to different conformational states which are less active but more stable.

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页码：489 / 493

页数：5

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