THE FLUOROMETRIC-DETERMINATION OF EUROPIUM ION CONCENTRATION AS USED IN TIME-RESOLVED FLUOROIMMUNOASSAY

被引：21

作者：

DAKUBU, S

EKINS, RP

机构：

来源：

ANALYTICAL BIOCHEMISTRY | 1985年 / 144卷 / 01期

关键词：

D O I：

10.1016/0003-2697(85)90078-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A model of the relationships between optimal fluorescence yield, Eu3+ concentration and the concentration of .beta.-diketone in the determination of Eu3+ concentration in aqueous solutions as used in time-resolved fluoroimmunoassays was developed. The model, in addition to optimizing the determination of the metal ion, also presents a new simple and rapid method for the determination of stability constants for the formation of .beta.-diketone-Eu3+ complexes. It was shown how the method may be extended to determine the formation constants for Eu3+ with other chelates.

引用

页码：20 / 26

页数：7

共 14 条

[1]

DAKUBU S, 1984, PRACTICAL IMMUNOASSA

[2] CALCIUM AND POTASSIUM LEVELS IN LEAVES MEASURED BY X-RAY-FLUORESCENCE ANALYSIS [J].