NT-3 AND BDNF PROTECT CNS NEURONS AGAINST METABOLIC EXCITOTOXIC INSULTS

被引：352

作者：

CHENG, B ^{[1
]}

MATTSON, MP ^{[1
]}

机构：

[1] UNIV KENTUCKY, DEPT ANAT & NEUROBIOL, LEXINGTON, KY 40536 USA

来源：

BRAIN RESEARCH | 1994年 / 640卷 / 1-2期

关键词：

CALCIUM; EXCITOTOXICITY; FURA-2; NEUROTROPHIN; TYROSINE KINASE; TRKB; TRKC;

D O I：

10.1016/0006-8993(94)91857-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) were recently shown to have biological activity in central neurons. In the present study, NT-3 and BDNF attenuated glucose deprivation-induced neuronal damage dose-dependently in rat hippocampal, septal and cortical cultures. Direct measurements of intraneuronal free calcium levels ([Ca2+]i) and manipulations of calcium influx demonstrated that NT-3 and BDNF each prevented the elevation of [Ca2+]i, that mediated glucose deprivation-induced injury. Studies in cultures depleted of glia indicated a direct action of NT-3 and BDNF on neurons. Neurons pretreated with NT-3 or BDNF for 24 hr were more resistant to glutamate neurotoxicity, and showed attenuated [Ca2+]i responses to glutamate. TrkB (BDNF receptor) and trkC (NT-3 receptor) proteins were present in hippocampal, cortical and septal cultures where they were localized to neuronal cell bodies and neurites. The data demonstrate that NT-3 and BDNF can protect neurons against metabolic and excitotoxic insults, and suggest that these neurotrophins may serve [Ca2+]i-stabilizing and neuroprotective functions in the brain.

引用

页码：56 / 67

页数：12

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