Time course of oxysterol formation during in vitro oxidation of low density lipoprotein

被引：73

作者：

Dzeletovic, S ^{[1
]}

Babiker, A ^{[1
]}

Lund, E ^{[1
]}

Diczfalusy, U ^{[1
]}

机构：

[1] KAROLINSKA INST,HUDDINGE UNIV HOSP,DIV CLIN CHEM,DEPT MED LAB SCI & TECHNOL,S-14186 HUDDINGE,SWEDEN

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 1995年 / 78卷 / 02期

关键词：

soybean lipoxygenase; copper oxidation; cholesterol; cholesterol-7-hydroperoxides; fatty acids;

D O I：

10.1016/0009-3084(95)02489-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol oxidation products (oxysterols) have been implicated in several aspects of atherogenesis; they affect key enzymes in cholesterol homeostasis, induce calcification in vascular cells and possess cytotoxic properties. Oxysterols are formed during oxidative modification of low density lipoprotein (LDL). Using a recently developed method based on isotope dilution-mass spectrometry, the kinetics of formation of oxysterols during oxidation of LDL by cupric ions or soybean lipoxygenase was studied. The same products, mainly 7- and 5-oxygenated cholesterol, were formed by the two oxidation methods. Virtually no side-chain oxidized oxysterols were formed. During the oxidations, preferentially esterified cholesterol was consumed and consumption of polyunsaturated fatty acids and formation of conjugated dienes preceded the appearance of oxysterols. Cholesterol 7-hydroperoxides potential cytotoxins, were present in LDL oxidized by copper or lipoxygenase.

引用

页码：119 / 128

页数：10

共 34 条

[1] THE MECHANISMS OF THE REARRANGEMENTS OF ALLYLIC HYDROPEROXIDES - 5-ALPHA-HYDROPEROXY-3-BETA-HYDROXYCHOLEST-6-ENE AND 7-ALPHA-HYDROPEROXY-3-BETA-HYDROXYCHOLEST-5-ENE [J].