HIGH LACCASE PRODUCING MUTANTS OF PLEUROTUS FLORIDA

被引：10

作者：

DHALIWAL, RPS ^{[1
]}

GARCHA, HS ^{[1
]}

KHANNA, PK ^{[1
]}

机构：

[1] PUNJAB AGR UNIV,DEPT MICROBIOL,LUDHIANA 141004,PUNJAB,INDIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 01期

关键词：

PLEUROTUS FLORIDA; LACCASE; PARA-CONSTITUTIVE; PROTOPLASTS; MUTAGENESIS;

D O I：

10.1007/BF01200681

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pleurotus florida produced high amounts of laccase (4.60 U/ml) in malt extract broth after 12 days' growth under stationary conditions. The production of laccase was semi-constitutive. Hyperlaccase mutants of P. florida were obtained through mutagenesis of mycelial protoplasts using N-methyl-N'-nitro-N-nitrosoguanidine (50-mu-g/ml) for 2 min. Three hyperlaccase mutants were selected showing growth and enzyme production responses similar to the parent.

引用

页码：39 / 41

页数：3

共 8 条

[1]

De Vries O.M.H., Kooistra W.H.C.F., Wessells J.G.H., Formation of an extracellular laccase by a Schizophyllum commune dikaryon, Journal of General Microbiology, 132, pp. 2817-2826, (1986)

[2]

Iijima Y., Yanagi S.O., A method for high yield preparation of and high frequency regeneration of basidiomycete Pleurotus ostreatus (‘Hiratake’) protoplasts using sulfite pulp waste components, Agricultural and Biological Chemistry, 50, pp. 1855-1861, (1986)

[3]

Kirk T.K., Farrell R.L., Enzymatic combustion: the microbial degradation of lignin, Annual Review of Microbiology, 41, pp. 465-505, (1987)

[4]

Liesola M.S.A., Ulmer D.C., Woldner R., Fiechter A., Role of veratryl alcohol in lignin degradation by Phanerochaete chrysosporium, Journal of Biotechnology, 1, pp. 331-339, (1984)

[5]

Mukherjee M., Sen Gupta S., Mutagenesis of protoplasts and regeneration of mycelium in the mushroom Volvariella volvacea, Applied and Environmental Microbiology, 56, pp. 1412-1414, (1986)

[6]

Schoemaker H.E., Leisola M.S.A., Degradation of lignin by Phanerochaete chrysosporium, Journal of Biotechnology, 13, pp. 101-109, (1990)

[7]

Singh R.P., Garcha H.S., Khanna P.K., Laccase production by Pleurotus spp., Indian Journal of Microbiology, 28, pp. 38-41, (1988)

[8]

Ulmer D.C., Leisola M.S.A., Fiechter A., Possible induction of ligninolytic system of Phanerochaete chrysosporium, Journal of Biotechnology, 18, pp. 153-157, (1984)

← 1 →